Temperature-sensitive mutants of reovirus type 3: Studies on the synthesis of viral peptides

The synthesis of the major reovirus peptides has been examined in cells infected with ts mutants containing lesions in five cistrons. Immunological precipitation has been used so that peptides made in small amounts could be detected. At the permissive temperature, the mutants did not significantly differ from the wild type. At nonpermissive temperatures, different mutants produced different amounts of viral peptides, but all of the major peptides were made in the mutants examined and the size of each of these peptides was the same as in the wild type.