Temperature-sensitive PSII and promiscuous PSI as a possible solution for sustainable photosynthetic hydrogen production

Yuval Mazor, Hila Toporik, Nathan Nelson*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


Sustainable hydrogen production in cyanobacteria becomes feasible as a result of our recent studies of the structure of photosystem I encoding operon in a marine phage. We demonstrated that the fused PsaJF subunit from the phage, substituted for the two separate subunits in Synechocystis, enabled the mutated PSI to accept electrons from additional electron donors such as respiratory cytochromes. In this way, a type of photorespiration was created in which the cell consumes organic material through respiratory processes and PSI serves as a terminal electron acceptor, substituting for cytochrome oxidase. We designed a hydrogen-producing bioreactor in which this type of photorespiration could utilize the organic material of the cell as an electron source for H2 production. We propose, in parallel, to engineer cyanobacterial and/or algal strains with a temperature-sensitive PSII and enhanced respiration rates to achieve efficient and sustainable hydrogen production. This article is part of a Special Issue entitled: Photosynthesis Research for Sustainability: from Natural to Artificial.

Original languageAmerican English
Pages (from-to)1122-1126
Number of pages5
JournalBiochimica et Biophysica Acta - Bioenergetics
Issue number8
StatePublished - Aug 2012
Externally publishedYes


  • Bioreactor
  • Cyanobacterium
  • Electron transfer
  • Hydrogen production
  • Micro-alga
  • Photosynthetic reaction center


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