TY - JOUR
T1 - Template boundary in a yeast telomerase specified by RNA structure
AU - Tzfati, Yehuda
AU - Fulton, Tracy B.
AU - Roy, Jagoree
AU - Blackburn, Elizabeth H.
PY - 2000/5/5
Y1 - 2000/5/5
N2 - The telomerase ribonucleoprotein has a phylogenetically divergent RNA subunit, which contains a short template for telomeric DNA synthesis. To understand how telomerase RNA participates in mechanistic aspects of telomere synthesis, we studied a conserved secondary structure adjacent to the template. Disruption of this structure caused DNA synthesis to proceed beyond the normal template boundary, resulting in altered telomere sequences, telomere shortening, and cellular growth defects. Compensatory mutations restored normal telomerase function. Thus, the RNA structure, rather than its sequence, specifies the template boundary. This study reveals a specific function for an RNA structure in the enzymatic action of telomerase.
AB - The telomerase ribonucleoprotein has a phylogenetically divergent RNA subunit, which contains a short template for telomeric DNA synthesis. To understand how telomerase RNA participates in mechanistic aspects of telomere synthesis, we studied a conserved secondary structure adjacent to the template. Disruption of this structure caused DNA synthesis to proceed beyond the normal template boundary, resulting in altered telomere sequences, telomere shortening, and cellular growth defects. Compensatory mutations restored normal telomerase function. Thus, the RNA structure, rather than its sequence, specifies the template boundary. This study reveals a specific function for an RNA structure in the enzymatic action of telomerase.
UR - http://www.scopus.com/inward/record.url?scp=0034608073&partnerID=8YFLogxK
U2 - 10.1126/science.288.5467.863
DO - 10.1126/science.288.5467.863
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C2 - 10797010
AN - SCOPUS:0034608073
SN - 0036-8075
VL - 288
SP - 863
EP - 867
JO - Science
JF - Science
IS - 5467
ER -