TY - JOUR
T1 - Tepidibacillus infernus sp. Nov., a moderately thermophilic, selenate-and arsenate-respiring hydrolytic bacterium isolated from a gold mine, and emended description of the genus Tepidibacillus
AU - Podosokorskaya, Olga A.
AU - Merkel, Alexander Y.
AU - Gavrilov, Sergey N.
AU - Fedoseev, Igor
AU - Van Heerden, Esta
AU - Cason, Errol D.
AU - Novikov, Andrey A.
AU - Kolganova, Tatyana V.
AU - Korzhenkov, Aleksei A.
AU - Bonch-Osmolovskaya, Elizaveta A.
AU - Kublanov, Ilya V.
N1 - Publisher Copyright:
© 2016 IUMS.
PY - 2016/8
Y1 - 2016/8
N2 - A novel aerotolerant anaerobic, moderately thermophilic, organotrophic bacterium, strain MBL-TLPT, was isolated from a sample of microbial mat, developed under the flow of subsurface water in TauTona gold mine, South Africa. Cells of the new isolate were flagellated, spore-forming rods, 0.25-0.5 μm in width and 3-15 μm in length. Strain MBL-TLPT grew in the temperature range from 25 to 58 °C, pH range from 5.6 to 8.8 and at NaCl concentration from 0 to 85 g l−1. The isolate was able to ferment yeast extract and mono-, oligo-and polysaccharides, including starch and xanthan gum. The G+C content of the DNA was 35 mol%. Phylogenetic analysis of 16S rRNA gene sequences of strain MBL-TLPT and relatives showed its affiliation to the genus Tepidibacillus. Tepidibacillus fermentans STGHT was its closest relative (97.1% identity of 16S rRNA gene sequences). Based on phylogenetic analysis and the physiological properties of the novel isolate, we propose a novel species, Tepidibacillus infernus sp. nov., with MBL-TLPT(=DSM 28123T=VKM B-2949T) as the type strain.
AB - A novel aerotolerant anaerobic, moderately thermophilic, organotrophic bacterium, strain MBL-TLPT, was isolated from a sample of microbial mat, developed under the flow of subsurface water in TauTona gold mine, South Africa. Cells of the new isolate were flagellated, spore-forming rods, 0.25-0.5 μm in width and 3-15 μm in length. Strain MBL-TLPT grew in the temperature range from 25 to 58 °C, pH range from 5.6 to 8.8 and at NaCl concentration from 0 to 85 g l−1. The isolate was able to ferment yeast extract and mono-, oligo-and polysaccharides, including starch and xanthan gum. The G+C content of the DNA was 35 mol%. Phylogenetic analysis of 16S rRNA gene sequences of strain MBL-TLPT and relatives showed its affiliation to the genus Tepidibacillus. Tepidibacillus fermentans STGHT was its closest relative (97.1% identity of 16S rRNA gene sequences). Based on phylogenetic analysis and the physiological properties of the novel isolate, we propose a novel species, Tepidibacillus infernus sp. nov., with MBL-TLPT(=DSM 28123T=VKM B-2949T) as the type strain.
KW - Anaerobes
KW - Deep subsurface
KW - Gold mines
KW - Hydrolytics
KW - Respiration with exogenic electron acceptors
KW - Thermophiles
UR - http://www.scopus.com/inward/record.url?scp=84988360139&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.001166
DO - 10.1099/ijsem.0.001166
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C2 - 27216447
AN - SCOPUS:84988360139
SN - 1466-5026
VL - 66
SP - 3189
EP - 3194
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 8
M1 - 001166
ER -