Tepidiforma bonchosmolovskayae gen. Nov., sp. nov., a moderately thermophilic chloroflexi bacterium from a chukotka hot spring (arctic, russia), representing a novel class, tepidiformia, which includes the previously uncultivated lineage olb14

A novel aerobic moderately thermophilic bacterium, strain 3753O^T, was isolated from a Chukotka hot spring (Arctic, Russia) using the newly developed technology of laser engineering of microbial systems. Сells were regular short rods, 0.4×0.8–2.0 µm in size, with a monoderm-type envelope and a single flagellum. The temperature and pH ranges for growth were 42–60 °C and pH 6.5–8.5, the optima being 50–54 °C and pH 7.3. Strain 3753O^T grew chemoorganoheterotrophically on a number of carbohydrates or peptidic substrates and volatile fatty acids, and chemolithoautotrophically with siderite (FeCO₃) as the electron donor. The major cellular fatty acid was branched C_19:0. Phosphatidylethanolamine, phosphatidylglycerol and two unidentified phos-pholipids as well as two yellow carotenoid-type pigments were detected in the polar lipid extract. Strain 3753O^T was inhibited by chloramphenicol, polymyxin B, vancomycin, streptomycin, neomycin and kanamycin, but resistant to the action of novobiocin and ampicillin. The DNA G+C content was 69.9 mol%. The 16S rRNA gene as well as 51 conservative protein sequence-based phylogenetic analyses placed strain 3753O^T within the previously uncultivated lineage OLB14 in the phylum Chloroflexi. Taking into account the phylogenetic position as well as phenotypic properties of the novel isolate, the novel genus and species Tepidiforma bonchosmolovskayae gen. nov., sp. nov., within the Tepidiformaceae fam. nov., the Tepidiformales ord. nov. and the Tepidiformia classis nov. are proposed. The type strain of Tepidiforma bonchosmolovskayae is 3753O^T (=VKM B-3389^T=KTCT 72284^T).