Testing and characterizing the simple carrier

W. R. Lieb*, W. D. Stein

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

125 Scopus citations

Abstract

1. 1. We treat the simple carrier at the level of steady-state analysis, making no assumptions as to equilibrium or symmetry and taking into account specifically the effect of electric fields and unstirred layers. 2. 2. We develop tests and rejection criteria applicable under these conditions. 3. 3. For those transport systems which pass the tests for the simple carrier, we show how to obtain from the experimental data (i) the permeabilities of any unstirred layers which may be present at the membrane surfaces and (ii) a complete characterization of the transport system itself in terms of only four independent parameters. 4. 4. The interpretation of these parameters in terms of molecular rate constants is discussed.

Original languageEnglish
Pages (from-to)178-196
Number of pages19
JournalBiochimica et Biophysica Acta - Biomembranes
Volume373
Issue number2
DOIs
StatePublished - 10 Dec 1974

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