TY - JOUR
T1 - Testing and characterizing the simple pore
AU - Lieb, W. R.
AU - Stein, W. D.
PY - 1974/12/10
Y1 - 1974/12/10
N2 - 1. 1. We define a simple (or singly-occupied) pore and show that any pore which is fully occupied by a single substrate particle behaves as a simple pore at the level of steady-state analysis. 2. 2. The analysis is independent of assumptions as to (a) symmetry, (b) equilibrium, (c) the distribution of electrical potential in the membrane, and (d) the presence or absence of unstirred layers. 3. 3. We suggest simple experimental procedures involving only one substrate, the results of which can be used to test for and characterize the simple pore. For ions, only electrical measurements are needed.
AB - 1. 1. We define a simple (or singly-occupied) pore and show that any pore which is fully occupied by a single substrate particle behaves as a simple pore at the level of steady-state analysis. 2. 2. The analysis is independent of assumptions as to (a) symmetry, (b) equilibrium, (c) the distribution of electrical potential in the membrane, and (d) the presence or absence of unstirred layers. 3. 3. We suggest simple experimental procedures involving only one substrate, the results of which can be used to test for and characterize the simple pore. For ions, only electrical measurements are needed.
UR - http://www.scopus.com/inward/record.url?scp=0016235972&partnerID=8YFLogxK
U2 - 10.1016/0005-2736(74)90143-6
DO - 10.1016/0005-2736(74)90143-6
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C2 - 4429732
AN - SCOPUS:0016235972
SN - 0005-2736
VL - 373
SP - 165
EP - 177
JO - Biochimica et Biophysica Acta - Biomembranes
JF - Biochimica et Biophysica Acta - Biomembranes
IS - 2
ER -