Testosterone skin permeation enhancement by menthol through formation of eutectic with drug and interaction with skin lipids

Yael Kaplun-Frischoff*, Elka Touitou

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

119 Scopus citations

Abstract

The aim of this investigation was to elucidate the mechanism of skin permeation enhancement of the lipophilic drug testosterone, by menthol. Menthol was found to form eutectic mixtures with testosterone, cholesteryl oleate, and ceramides. DSC measurements showed that menthol drastically lowers the T(m) of testosterone, from 153.7 to.39.9 °C. The decrease in T(m) resulted in an increase in the solubility of testosterone in an aqueous ethanol vehicle by 2.8-fold, which caused a corresponding 2.8-fold increase in the flux of testosterone. A further increase in skin flux, to eight times the base line, could be attributed to the effect of menthol on the skin barrier properties. This assumption is supported by DSC results showing that menthol decreases the T(m), of cholesteryl oleate and ceramides and modifies the thermogram profile of isolated stratum corneum. The results of this investigation indicate that menthol affects skin permeation by a dual mechanism: by forming a eutectic with the penetrating compound, thereby increasing its solubility, and by altering the barrier properties of the stratum corneum. Moreover, this study indicates that both types of interactions must be taken into consideration when using chemical enhancers and that decreasing the melting temperature of the permeant through formation of a eutectic could be one approach for increasing solubility and permeation rates.

Original languageEnglish
Pages (from-to)1394-1399
Number of pages6
JournalJournal of Pharmaceutical Sciences
Volume86
Issue number12
DOIs
StatePublished - Dec 1997

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