Tetrahedral intermediate in a specific α-chymotrypsin inhibitor complex detected by Laser Raman spectroscopy

George P. Hess; David Seybert; Aaron Lewis; John Spoonhower; Robert Cookingham

doi:10.1126/science.238285

Tetrahedral intermediate in a specific α-chymotrypsin inhibitor complex detected by Laser Raman spectroscopy

George P. Hess^*, David Seybert, Aaron Lewis, John Spoonhower, Robert Cookingham

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Laser Raman spectroscopy indicates that inhibition of α-chymotrypsin by 2-phenylethaneboronic acid occurs in two steps: (i) the formation of a loosely bound complex, in which the boron remains in a trigonal configuration; and (ii) reaction of the boron with a functional group in the catalytic site of the enzyme, resulting in a reversible, stable tetrahedral adduct.

Original language	English
Pages (from-to)	384-386
Number of pages	3
Journal	Science
Volume	189
Issue number	4200
DOIs	https://doi.org/10.1126/science.238285
State	Published - 1975
Externally published	Yes

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title = "Tetrahedral intermediate in a specific α-chymotrypsin inhibitor complex detected by Laser Raman spectroscopy",

abstract = "Laser Raman spectroscopy indicates that inhibition of α-chymotrypsin by 2-phenylethaneboronic acid occurs in two steps: (i) the formation of a loosely bound complex, in which the boron remains in a trigonal configuration; and (ii) reaction of the boron with a functional group in the catalytic site of the enzyme, resulting in a reversible, stable tetrahedral adduct.",

author = "Hess, {George P.} and David Seybert and Aaron Lewis and John Spoonhower and Robert Cookingham",

year = "1975",

doi = "10.1126/science.238285",

language = "אנגלית",

volume = "189",

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journal = "Science",

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publisher = "American Association for the Advancement of Science",

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AU - Hess, George P.

AU - Seybert, David

AU - Lewis, Aaron

AU - Spoonhower, John

AU - Cookingham, Robert

PY - 1975

Y1 - 1975

N2 - Laser Raman spectroscopy indicates that inhibition of α-chymotrypsin by 2-phenylethaneboronic acid occurs in two steps: (i) the formation of a loosely bound complex, in which the boron remains in a trigonal configuration; and (ii) reaction of the boron with a functional group in the catalytic site of the enzyme, resulting in a reversible, stable tetrahedral adduct.

AB - Laser Raman spectroscopy indicates that inhibition of α-chymotrypsin by 2-phenylethaneboronic acid occurs in two steps: (i) the formation of a loosely bound complex, in which the boron remains in a trigonal configuration; and (ii) reaction of the boron with a functional group in the catalytic site of the enzyme, resulting in a reversible, stable tetrahedral adduct.

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DO - 10.1126/science.238285

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C2 - 238285

AN - SCOPUS:0016760879

SN - 0036-8075

VL - 189

SP - 384

EP - 386

JO - Science

JF - Science

IS - 4200

ER -

Tetrahedral intermediate in a specific α-chymotrypsin inhibitor complex detected by Laser Raman spectroscopy

Abstract

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