The abp gene in Geobacillus stearothermophilus T-6 encodes a GH27 β-l-arabinopyranosidase

Rachel Salama; Onit Alalouf; Orly Tabachnikov; Gennady Zolotnitsky; Gil Shoham; Yuval Shoham

doi:10.1016/j.febslet.2012.05.062

The abp gene in Geobacillus stearothermophilus T-6 encodes a GH27 β-l-arabinopyranosidase

Rachel Salama, Onit Alalouf, Orly Tabachnikov, Gennady Zolotnitsky, Gil Shoham, Yuval Shoham^*

^*Corresponding author for this work

Institute of Chemistry

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

In this study we demonstrate that the abp gene in Geobacillus stearothermophilus T-6 encodes a family 27 glycoside hydrolase β-l-arabinopyranosidase. The catalytic constants towards the chromogenic substrate pNP-β-l-arabinopyranoside were 0.8 ± 0.1 mM, 6.6 ± 0.3 s ^-1, and 8.2 ± 0.3 s ^-1 mM ^-1 for K _m, k _cat and k _cat/K _m, respectively. ¹³C NMR spectroscopy unequivocally showed that Abp is capable of removing β-l-arabinopyranose residues from the natural arabino- polysaccharide, larch arabinogalactan. Most family 27 enzymes are active on galactose and contain a conserved Asp residue, whereas in Abp this residue is Ile67, which shifts the specificity of the enzyme towards arabinopyranoside. Crown

Original language	English
Pages (from-to)	2436-2442
Number of pages	7
Journal	FEBS Letters
Volume	586
Issue number	16
DOIs	https://doi.org/10.1016/j.febslet.2012.05.062
State	Published - 30 Jul 2012

Bibliographical note

Funding Information:
This work was supported by the Israel Science Foundation Grant 500/10 (to Y. S.), United States-Israel Binational Science Foundation (BSF; Jerusalem, Israel) Grant 96 178 (to Y. S.), the I-CORE Program of the Planning and Budgeting Committee and The Israel Science Foundation (grant No 152/11), the Ministry of Environmental Protection, Grand Technion Energy Program (GTEP) and the Helmsley Charity Fund.

Keywords

Arabinopyranose
C NMR
Glycoside hydrolase family 27
Larch arabinogalactan
Sugar beet arabinan
β-l-Arabinopyranosidase

Access to Document

10.1016/j.febslet.2012.05.062

Cite this

@article{a4d3a4307cec443f8d8dea1e77e8c8f3,

title = "The abp gene in Geobacillus stearothermophilus T-6 encodes a GH27 β-l-arabinopyranosidase",

abstract = "In this study we demonstrate that the abp gene in Geobacillus stearothermophilus T-6 encodes a family 27 glycoside hydrolase β-l-arabinopyranosidase. The catalytic constants towards the chromogenic substrate pNP-β-l-arabinopyranoside were 0.8 ± 0.1 mM, 6.6 ± 0.3 s -1, and 8.2 ± 0.3 s -1 mM -1 for K m, k cat and k cat/K m, respectively. 13C NMR spectroscopy unequivocally showed that Abp is capable of removing β-l-arabinopyranose residues from the natural arabino- polysaccharide, larch arabinogalactan. Most family 27 enzymes are active on galactose and contain a conserved Asp residue, whereas in Abp this residue is Ile67, which shifts the specificity of the enzyme towards arabinopyranoside. Crown",

keywords = "Arabinopyranose, C NMR, Glycoside hydrolase family 27, Larch arabinogalactan, Sugar beet arabinan, β-l-Arabinopyranosidase",

author = "Rachel Salama and Onit Alalouf and Orly Tabachnikov and Gennady Zolotnitsky and Gil Shoham and Yuval Shoham",

note = "Funding Information: This work was supported by the Israel Science Foundation Grant 500/10 (to Y. S.), United States-Israel Binational Science Foundation (BSF; Jerusalem, Israel) Grant 96 178 (to Y. S.), the I-CORE Program of the Planning and Budgeting Committee and The Israel Science Foundation (grant No 152/11), the Ministry of Environmental Protection, Grand Technion Energy Program (GTEP) and the Helmsley Charity Fund. ",

year = "2012",

month = jul,

day = "30",

doi = "10.1016/j.febslet.2012.05.062",

language = "אנגלית",

volume = "586",

pages = "2436--2442",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc.",

number = "16",

}

TY - JOUR

T1 - The abp gene in Geobacillus stearothermophilus T-6 encodes a GH27 β-l-arabinopyranosidase

AU - Salama, Rachel

AU - Alalouf, Onit

AU - Tabachnikov, Orly

AU - Zolotnitsky, Gennady

AU - Shoham, Gil

AU - Shoham, Yuval

N1 - Funding Information: This work was supported by the Israel Science Foundation Grant 500/10 (to Y. S.), United States-Israel Binational Science Foundation (BSF; Jerusalem, Israel) Grant 96 178 (to Y. S.), the I-CORE Program of the Planning and Budgeting Committee and The Israel Science Foundation (grant No 152/11), the Ministry of Environmental Protection, Grand Technion Energy Program (GTEP) and the Helmsley Charity Fund.

PY - 2012/7/30

Y1 - 2012/7/30

N2 - In this study we demonstrate that the abp gene in Geobacillus stearothermophilus T-6 encodes a family 27 glycoside hydrolase β-l-arabinopyranosidase. The catalytic constants towards the chromogenic substrate pNP-β-l-arabinopyranoside were 0.8 ± 0.1 mM, 6.6 ± 0.3 s -1, and 8.2 ± 0.3 s -1 mM -1 for K m, k cat and k cat/K m, respectively. 13C NMR spectroscopy unequivocally showed that Abp is capable of removing β-l-arabinopyranose residues from the natural arabino- polysaccharide, larch arabinogalactan. Most family 27 enzymes are active on galactose and contain a conserved Asp residue, whereas in Abp this residue is Ile67, which shifts the specificity of the enzyme towards arabinopyranoside. Crown

AB - In this study we demonstrate that the abp gene in Geobacillus stearothermophilus T-6 encodes a family 27 glycoside hydrolase β-l-arabinopyranosidase. The catalytic constants towards the chromogenic substrate pNP-β-l-arabinopyranoside were 0.8 ± 0.1 mM, 6.6 ± 0.3 s -1, and 8.2 ± 0.3 s -1 mM -1 for K m, k cat and k cat/K m, respectively. 13C NMR spectroscopy unequivocally showed that Abp is capable of removing β-l-arabinopyranose residues from the natural arabino- polysaccharide, larch arabinogalactan. Most family 27 enzymes are active on galactose and contain a conserved Asp residue, whereas in Abp this residue is Ile67, which shifts the specificity of the enzyme towards arabinopyranoside. Crown

KW - Arabinopyranose

KW - C NMR

KW - Glycoside hydrolase family 27

KW - Larch arabinogalactan

KW - Sugar beet arabinan

KW - β-l-Arabinopyranosidase

UR - http://www.scopus.com/inward/record.url?scp=84864286563&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2012.05.062

DO - 10.1016/j.febslet.2012.05.062

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 22687242

AN - SCOPUS:84864286563

SN - 0014-5793

VL - 586

SP - 2436

EP - 2442

JO - FEBS Letters

JF - FEBS Letters

IS - 16

ER -

The abp gene in Geobacillus stearothermophilus T-6 encodes a GH27 β-l-arabinopyranosidase

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Cite this