The activity of the TRP-like channel depends on its expression system

Shaya Lev, Ben Katz, Baruch Minke*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

The Drosophila light activated TRP and TRPL channels have been a model for TRPC channel gating. Several gating mechanisms have been proposed following experiments conducted on photoreceptor and tissue cultured cells. However, conclusive evidence for any mechanism is still lacking. Here, we show that the Drosophila TRPL channel expressed in tissue cultured cells is constitutively active in S2 cells but is silent in HEK cells. Modulations of TRPL channel activity in different expression system by pharmacology or specific enzymes, which change the lipid content of the plasma membrane, resulted in conflicting effects. These findings demonstrate the difficulty in elucidating TRPC gating, as channel behavior is expression system dependent. However, clues on the gating mechanism may arise from understanding how different expression systems affect TRPC channel activation.

Original languageEnglish
Pages (from-to)86-93
Number of pages8
JournalChannels
Volume6
Issue number2
DOIs
StatePublished - 2012

Keywords

  • Constitutive activity
  • Gating mechanism
  • Membrane lipids
  • TRP channels

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