The anatomical boundary of the rat claustrum

Christopher M. Dillingham, Mathias L. Mathiasen, Bethany E. Frost, Marie A.C. Lambert, Emma J. Bubb, Maciej M. Jankowski, John P. Aggleton, Shane M. O’Mara*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


The claustrum is a subcortical nucleus that exhibits dense connectivity across the neocortex. Considerable recent progress has been made in establishing its genetic and anatomical characteristics, however, a core, contentious issue that regularly presents in the literature pertains to the rostral extent of its anatomical boundary. The present study addresses this issue in the rat brain. Using a combination of immunohistochemistry and neuroanatomical tract tracing, we have examined the expression profiles of several genes that have previously been identified as exhibiting a differential expression profile in the claustrum relative to the surrounding cortex. The expression profiles of parvalbumin (PV), crystallin mu (Crym), and guanine nucleotide binding protein (G protein), gamma 2 (Gng2) were assessed immunohistochemically alongside, or in combination with cortical anterograde, or retrograde tracer injections. Retrograde tracer injections into various thalamic nuclei were used to further establish the rostral border of the claustrum. Expression of all three markers delineated a nuclear boundary that extended considerably (∼500 μm) beyond the anterior horn of the neostriatum. Cortical retrograde and anterograde tracer injections, respectively, revealed distributions of cortically-projecting claustral neurons and cortical efferent inputs to the claustrum that overlapped with the gene marker-derived claustrum boundary. Finally, retrograde tracer injections into the thalamus revealed insular cortico-thalamic projections encapsulating a claustral area with strongly diminished cell label, that extended rostral to the striatum.

Original languageAmerican English
Article number53
JournalFrontiers in Neuroanatomy
StatePublished - 3 Jun 2019

Bibliographical note

Publisher Copyright:
© 2019 Dillingham, Mathiasen, Frost, Lambert, Bubb, Jankowski, Aggleton and O’Mara.


  • Crystallin mu
  • Gng2
  • Immunofluorescence
  • Immunohistochemistry
  • Midline thalamus
  • Neuroanatomical tracing
  • Parvalbumin


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