TY - JOUR
T1 - The association of H‐2 antigens and EAC receptors on the surface of peritoneal cells
AU - Schlesinger, M.
AU - Chaouat, M.
PY - 1975/1
Y1 - 1975/1
N2 - The exposure of murine peritoneal cells to anti‐H‐2 sera results in a diminished expressoion of H‐2 antigen on the cell surface. Concomitant with this „H‐2 modulation” the capacity of macrphages to bind sheep red blood cells coated with antibody and complement (EAC) was markedly diminished. In contrast, there was no change in the capacity of modulated macrophages to bind sheep red blood cells coated with antibody alone (EA). Antibodies to K end H‐2 specificities were more effective in reducing the binding of EAC than antibodies to D end H‐2 specificities. Exposure of peritoneal cells to θ or Ly antisera had no effect on the formation of EAC rosettes. Exposure of pertioneal cells to anti‐H‐2 sera, under conditions which would not allow moudulation of H‐2 antigens, also prevented the reduction of EAC binding. Thus, the EAC receptors and H‐2 antigenic specificities seem to be closely related on the surface of peritoneal cells, but constitute distinct cell surface structures. Perliminary evidence indicates that vinblastine, a microtubule depolymerizing agent, may disrupt the close association of EAC receptors and H‐2 antigens. It is suggested that the association of EAC receptors and K end H‐2 determinants on the membrane of macrphages may have implications for the regulations of the immune response by H‐2‐linked Ir genes.
AB - The exposure of murine peritoneal cells to anti‐H‐2 sera results in a diminished expressoion of H‐2 antigen on the cell surface. Concomitant with this „H‐2 modulation” the capacity of macrphages to bind sheep red blood cells coated with antibody and complement (EAC) was markedly diminished. In contrast, there was no change in the capacity of modulated macrophages to bind sheep red blood cells coated with antibody alone (EA). Antibodies to K end H‐2 specificities were more effective in reducing the binding of EAC than antibodies to D end H‐2 specificities. Exposure of peritoneal cells to θ or Ly antisera had no effect on the formation of EAC rosettes. Exposure of pertioneal cells to anti‐H‐2 sera, under conditions which would not allow moudulation of H‐2 antigens, also prevented the reduction of EAC binding. Thus, the EAC receptors and H‐2 antigenic specificities seem to be closely related on the surface of peritoneal cells, but constitute distinct cell surface structures. Perliminary evidence indicates that vinblastine, a microtubule depolymerizing agent, may disrupt the close association of EAC receptors and H‐2 antigens. It is suggested that the association of EAC receptors and K end H‐2 determinants on the membrane of macrphages may have implications for the regulations of the immune response by H‐2‐linked Ir genes.
UR - http://www.scopus.com/inward/record.url?scp=0016438316&partnerID=8YFLogxK
U2 - 10.1002/eji.1830050107
DO - 10.1002/eji.1830050107
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C2 - 1234044
AN - SCOPUS:0016438316
SN - 0014-2980
VL - 5
SP - 27
EP - 32
JO - European Journal of Immunology
JF - European Journal of Immunology
IS - 1
ER -