The reductive capacity of rat tissue homogenates and body fluids was determined by cyclic voltammetric measurements. The reductive capacity of rat lung, liver and kidney homogenates was significantly reduced four days after total body γray irradiation with 5.5 Gy as compared to controls. In parallel, reduced ability of the irradiated organ homogenates to scavenge hydroxyl radicals and to destroy hydrogen peroxide was recorded. However, no difference in their superoxide dismutase activity was found. The possible use of cyclic voltammetry as a method for qualitative evaluation of the ability of biological tissues to cope with oxidative stress is discussed.
Bibliographical noteFunding Information:
This work was supported by a grant obtained from the “Authority for Research and development”, The Hebrew University of Jerusalem and by a grant obtained from the “Ministry of Health”. This study is part of the requirements for M.Sc. degree in the Hebrew university of Mr. Oren Tirosh.
- Cyclic voltammetry
- Reducing agents