The Biological Role of the Long Non-coding RNA LINK-A in Ovarian Carcinoma

Natalie Filippov-Levy; Ben Davidson; Reuven Reich

doi:10.21873/anticanres.14691

The Biological Role of the Long Non-coding RNA LINK-A in Ovarian Carcinoma

Natalie Filippov-Levy, Ben Davidson^*, Reuven Reich^*

^*Corresponding author for this work

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Aim: To analyze the biological role of the long non-coding RNA LINK-A. Materials and Methods: An 850-bp segment from the second exon of LINK-A was removed using the CRISPR/Cas9 system in OVCA433 ovarian serous carcinoma cells. Spheroid formation, migration, invasion, proliferation, matrix metalloproteinase (MMP) activity and expression of cell-signaling proteins were assessed in vitro. Results: OVCA433 cells with LINK-A deletion were more invasive (p=0.0008) but had reduced migration and MMP9 secretion compared to controls (p=0.003 and p=0.005, respectively). LINK-A deletion did not affect proliferation but induced phosphorylation of extracellular signal-regulated kinase (10-fold; p=0.005). LINK-A knock out additionally reduced spheroid formation. Conclusion: Added to our previous data from analysis of clinical specimens, LINK-A is likely to be a tumor suppressor.

Original language	English
Pages (from-to)	6677-6684
Number of pages	8
Journal	Anticancer Research
Volume	40
Issue number	12
DOIs	https://doi.org/10.21873/anticanres.14691
State	Published - Dec 2020

Bibliographical note

Publisher Copyright:
© 2020 International Institute of Anticancer Research. All rights reserved.

Keywords

CRISPR/Cas9
High-grade serous carcinoma
In vitro
LINK-A
Long non-coding RNA
Tumorigenesis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.21873/anticanres.14691

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title = "The Biological Role of the Long Non-coding RNA LINK-A in Ovarian Carcinoma",

abstract = "Aim: To analyze the biological role of the long non-coding RNA LINK-A. Materials and Methods: An 850-bp segment from the second exon of LINK-A was removed using the CRISPR/Cas9 system in OVCA433 ovarian serous carcinoma cells. Spheroid formation, migration, invasion, proliferation, matrix metalloproteinase (MMP) activity and expression of cell-signaling proteins were assessed in vitro. Results: OVCA433 cells with LINK-A deletion were more invasive (p=0.0008) but had reduced migration and MMP9 secretion compared to controls (p=0.003 and p=0.005, respectively). LINK-A deletion did not affect proliferation but induced phosphorylation of extracellular signal-regulated kinase (10-fold; p=0.005). LINK-A knock out additionally reduced spheroid formation. Conclusion: Added to our previous data from analysis of clinical specimens, LINK-A is likely to be a tumor suppressor.",

keywords = "CRISPR/Cas9, High-grade serous carcinoma, In vitro, LINK-A, Long non-coding RNA, Tumorigenesis",

author = "Natalie Filippov-Levy and Ben Davidson and Reuven Reich",

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AU - Davidson, Ben

AU - Reich, Reuven

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N2 - Aim: To analyze the biological role of the long non-coding RNA LINK-A. Materials and Methods: An 850-bp segment from the second exon of LINK-A was removed using the CRISPR/Cas9 system in OVCA433 ovarian serous carcinoma cells. Spheroid formation, migration, invasion, proliferation, matrix metalloproteinase (MMP) activity and expression of cell-signaling proteins were assessed in vitro. Results: OVCA433 cells with LINK-A deletion were more invasive (p=0.0008) but had reduced migration and MMP9 secretion compared to controls (p=0.003 and p=0.005, respectively). LINK-A deletion did not affect proliferation but induced phosphorylation of extracellular signal-regulated kinase (10-fold; p=0.005). LINK-A knock out additionally reduced spheroid formation. Conclusion: Added to our previous data from analysis of clinical specimens, LINK-A is likely to be a tumor suppressor.

AB - Aim: To analyze the biological role of the long non-coding RNA LINK-A. Materials and Methods: An 850-bp segment from the second exon of LINK-A was removed using the CRISPR/Cas9 system in OVCA433 ovarian serous carcinoma cells. Spheroid formation, migration, invasion, proliferation, matrix metalloproteinase (MMP) activity and expression of cell-signaling proteins were assessed in vitro. Results: OVCA433 cells with LINK-A deletion were more invasive (p=0.0008) but had reduced migration and MMP9 secretion compared to controls (p=0.003 and p=0.005, respectively). LINK-A deletion did not affect proliferation but induced phosphorylation of extracellular signal-regulated kinase (10-fold; p=0.005). LINK-A knock out additionally reduced spheroid formation. Conclusion: Added to our previous data from analysis of clinical specimens, LINK-A is likely to be a tumor suppressor.

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KW - High-grade serous carcinoma

KW - In vitro

KW - LINK-A

KW - Long non-coding RNA

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The Biological Role of the Long Non-coding RNA LINK-A in Ovarian Carcinoma

Abstract

Bibliographical note

Keywords

UN SDGs

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