The Bmp Gradient of the Zebrafish Gastrula Guides Migrating Lateral Cells by Regulating Cell-Cell Adhesion

Sophia von der Hardt, Jeroen Bakkers, Adi Inbal, Lara Carvalho, Lilianna Solnica-Krezel, Carl Philipp Heisenberg, Matthias Hammerschmidt*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

114 Scopus citations

Abstract

Background: Bone morphogenetic proteins (Bmps) are required for the specification of ventrolateral cell fates during embryonic dorsoventral patterning and for proper convergence and extension gastrulation movements, but the mechanisms underlying the latter role remained elusive. Results: Via bead implantations, we show that the Bmp gradient determines the direction of lateral mesodermal cell migration during dorsal convergence in the zebrafish gastrula. This effect is independent of its role during dorsoventral patterning and of noncanonical Wnt signaling. However, it requires Bmp signal transduction through Alk8 and Smad5 to negatively regulate Ca2+/Cadherin-dependent cell-cell adhesiveness. In vivo, converging mesodermal cells form lamellipodia that attach to adjacent cells. Bmp signaling diminishes the Cadherin-dependent stability of such contact points, thereby abrogating subsequent cell displacement during lamellipodial retraction. Conclusions: We propose that the ventral-to-dorsal Bmp gradient has an instructive role to establish a reverse gradient of cell-cell adhesiveness, thereby defining different migratory zones and directing lamellipodia-driven cell migrations during dorsal convergence in lateral regions of the zebrafish gastrula.

Original languageAmerican English
Pages (from-to)475-487
Number of pages13
JournalCurrent Biology
Volume17
Issue number6
DOIs
StatePublished - 20 Mar 2007
Externally publishedYes

Bibliographical note

Funding Information:
We thank C. Niessen, M. Brand, E. De Robertis, M. Ekker, M. Hibi, J.-S. Joly, R. Kemler, S. Schulte-Merker, E. Weinberg, and L. Zon for sending plasmids and N. Birkner for help with real-time PCR. Work in M.H.'s laboratory was supported by the Max-Planck Society and the Human Frontier Science Program (Research Grant RGP9/2003). Work in L.S.-K.'s lab was supported by NIH grant GM55101.

Keywords

  • DEVBIO

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