The C-terminal domain of the HIV-1 Vif protein is natively unfolded in its unbound state

Tali H. Reingewertz, Hadar Benyamini, Mario Lebendiker, Deborah E. Shalev, Assaf Friedler*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

The human immunodeficiency virus type-1 (HIV-1) Vif protein neutralizes the cellular defense mechanism against the virus. The C-terminal domain of Vif (CTD, residues 141-192) mediates many of its interactions. Full-length Vif is difficult to purify in large amounts, hence the only available structure of Vif is of residues 140-155 within the ElonginBC complex. Other structural information, derived from modeling and indirect experiments, indicates that the Vif CTD may be unstructured. Here, we chemically synthesized the Vif CTD using pseudo-proline-building blocks, studied its solution structure in the unbound state using biophysical techniques and found that it is unstructured under physiological conditions. The circular dichroism (CD) spectrum of Vif CTD showed a pattern of random coil with residual helical structure. The 15N-HSQC nuclear magnetic resonance (NMR) spectrum was characteristic of natively unfolded peptides. Vif CTD eluted from an analytical gel filtration column earlier than expected, indicating an extended conformation. Disorder predictions found the CTD to be unstructured, in agreement with our experimental results. CD experiments showed that Vif CTD underwent a conformational change upon interacting with membrane-mimicking DPC micelles, but not upon binding to a peptide derived from its binding region in ElonginC. Our results provide direct evidence for the unfolded structure of the free Vif CTD and indicate that it may gain structure upon binding its natural ligands.

Original languageAmerican English
Pages (from-to)281-287
Number of pages7
JournalProtein Engineering, Design and Selection
Volume22
Issue number5
DOIs
StatePublished - May 2009

Bibliographical note

Funding Information:
This work was supported by the Israeli Ministry of Health and by the US – Israel Binational Science Foundation (BSF). T.H.R. was partly supported by the ISEF Foundation. H. B. was supported by a post-doctoral fellowship from the Lady Davis Fellowship Trust.

Keywords

  • Biophysics
  • HIV-1
  • Natively unfolded proteins
  • Peptides
  • Vif

Fingerprint

Dive into the research topics of 'The C-terminal domain of the HIV-1 Vif protein is natively unfolded in its unbound state'. Together they form a unique fingerprint.

Cite this