The calmodulin-binding transcription factor SIGNAL RESPONSIVE1 is a novel regulator of glucosinolate metabolism and herbivory tolerance in arabidopsis

K. Laluk, K. V.S.K. Prasad, T. Savchenko, H. Celesnik, K. Dehesh, M. Levy, T. Mitchell-Olds, A. S.N. Reddy*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

59 Scopus citations

Abstract

The Arabidopsis Ca2+/calmodulin (CaM)-binding transcription factor SIGNAL RESPONSIVE1 (AtSR1/CAMTA3) was previously identified as a key negative regulator of plant immune responses. Here, we report a new role for AtSR1 as a critical component of plant defense against insect herbivory. Loss of AtSR1 function impairs tolerance to feeding by the generalist herbivore Trichoplusia ni as well as wound-induced jasmonate accumulation. The susceptibility of the atsr1 mutant is associated with decreased total glucosinolate (GS) levels. The two key herbivory deterrents, indol-3-ylmethyl (I3M) and 4-methylsulfinylbutyl (4MSOB), showed the most significant reductions in atsr1 plants. Further, changes in AtSR1 transcript levels led to altered expression of several genes involved in GS metabolism including IQD1, MYB51 and AtST5a. Overall, our results establish AtSR1 as an important component of plant resistance to insect herbivory as well as one of only three described proteins involved in Ca2+/CaM-dependent signaling to function in the regulation of GS metabolism, providing a novel avenue for future investigations of plant-insect interactions.

Original languageAmerican English
Pages (from-to)2008-2015
Number of pages8
JournalPlant and Cell Physiology
Volume53
Issue number12
DOIs
StatePublished - Dec 2012

Bibliographical note

Funding Information:
This work was supported by the National Science Foundation [5333470 to A.S.N.R.], the Binational Agricultural Research and Development Fund [5357100 to A.S.N.R. and M.L.]; the National Institute of Health [R01 GM086496 to T.M.-O.].

Keywords

  • Arabidopsis
  • AtSR1
  • Glucosinolates
  • Herbivory
  • Wound responses

Fingerprint

Dive into the research topics of 'The calmodulin-binding transcription factor SIGNAL RESPONSIVE1 is a novel regulator of glucosinolate metabolism and herbivory tolerance in arabidopsis'. Together they form a unique fingerprint.

Cite this