The calmodulin-binding transcription factor SIGNAL RESPONSIVE1 is a novel regulator of glucosinolate metabolism and herbivory tolerance in arabidopsis

K. Laluk; K. V.S.K. Prasad; T. Savchenko; H. Celesnik; K. Dehesh; M. Levy; T. Mitchell-Olds; A. S.N. Reddy

doi:10.1093/pcp/pcs143

The calmodulin-binding transcription factor SIGNAL RESPONSIVE1 is a novel regulator of glucosinolate metabolism and herbivory tolerance in arabidopsis

K. Laluk, K. V.S.K. Prasad, T. Savchenko, H. Celesnik, K. Dehesh, M. Levy, T. Mitchell-Olds, A. S.N. Reddy^*

^*Corresponding author for this work

Department of Plant Pathology and Microbiology

Research output: Contribution to journal › Article › peer-review

60 Scopus citations

Abstract

The Arabidopsis Ca²⁺/calmodulin (CaM)-binding transcription factor SIGNAL RESPONSIVE1 (AtSR1/CAMTA3) was previously identified as a key negative regulator of plant immune responses. Here, we report a new role for AtSR1 as a critical component of plant defense against insect herbivory. Loss of AtSR1 function impairs tolerance to feeding by the generalist herbivore Trichoplusia ni as well as wound-induced jasmonate accumulation. The susceptibility of the atsr1 mutant is associated with decreased total glucosinolate (GS) levels. The two key herbivory deterrents, indol-3-ylmethyl (I3M) and 4-methylsulfinylbutyl (4MSOB), showed the most significant reductions in atsr1 plants. Further, changes in AtSR1 transcript levels led to altered expression of several genes involved in GS metabolism including IQD1, MYB51 and AtST5a. Overall, our results establish AtSR1 as an important component of plant resistance to insect herbivory as well as one of only three described proteins involved in Ca²⁺/CaM-dependent signaling to function in the regulation of GS metabolism, providing a novel avenue for future investigations of plant-insect interactions.

Original language	English
Pages (from-to)	2008-2015
Number of pages	8
Journal	Plant and Cell Physiology
Volume	53
Issue number	12
DOIs	https://doi.org/10.1093/pcp/pcs143
State	Published - Dec 2012

Bibliographical note

Funding Information:
This work was supported by the National Science Foundation [5333470 to A.S.N.R.], the Binational Agricultural Research and Development Fund [5357100 to A.S.N.R. and M.L.]; the National Institute of Health [R01 GM086496 to T.M.-O.].

Keywords

Arabidopsis
AtSR1
Glucosinolates
Herbivory
Wound responses

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title = "The calmodulin-binding transcription factor SIGNAL RESPONSIVE1 is a novel regulator of glucosinolate metabolism and herbivory tolerance in arabidopsis",

abstract = "The Arabidopsis Ca2+/calmodulin (CaM)-binding transcription factor SIGNAL RESPONSIVE1 (AtSR1/CAMTA3) was previously identified as a key negative regulator of plant immune responses. Here, we report a new role for AtSR1 as a critical component of plant defense against insect herbivory. Loss of AtSR1 function impairs tolerance to feeding by the generalist herbivore Trichoplusia ni as well as wound-induced jasmonate accumulation. The susceptibility of the atsr1 mutant is associated with decreased total glucosinolate (GS) levels. The two key herbivory deterrents, indol-3-ylmethyl (I3M) and 4-methylsulfinylbutyl (4MSOB), showed the most significant reductions in atsr1 plants. Further, changes in AtSR1 transcript levels led to altered expression of several genes involved in GS metabolism including IQD1, MYB51 and AtST5a. Overall, our results establish AtSR1 as an important component of plant resistance to insect herbivory as well as one of only three described proteins involved in Ca2+/CaM-dependent signaling to function in the regulation of GS metabolism, providing a novel avenue for future investigations of plant-insect interactions.",

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author = "K. Laluk and Prasad, {K. V.S.K.} and T. Savchenko and H. Celesnik and K. Dehesh and M. Levy and T. Mitchell-Olds and Reddy, {A. S.N.}",

note = "Funding Information: This work was supported by the National Science Foundation [5333470 to A.S.N.R.], the Binational Agricultural Research and Development Fund [5357100 to A.S.N.R. and M.L.]; the National Institute of Health [R01 GM086496 to T.M.-O.].",

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AU - Prasad, K. V.S.K.

AU - Savchenko, T.

AU - Celesnik, H.

AU - Dehesh, K.

AU - Levy, M.

AU - Mitchell-Olds, T.

AU - Reddy, A. S.N.

N1 - Funding Information: This work was supported by the National Science Foundation [5333470 to A.S.N.R.], the Binational Agricultural Research and Development Fund [5357100 to A.S.N.R. and M.L.]; the National Institute of Health [R01 GM086496 to T.M.-O.].

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The calmodulin-binding transcription factor SIGNAL RESPONSIVE1 is a novel regulator of glucosinolate metabolism and herbivory tolerance in arabidopsis

Abstract

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Keywords

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