Abstract
DNA damage presents a barrier to DNA-templated biochemical processes, including gene expression and faithful DNA replication. Compromised DNA repair leads to mutations, enhancing the risk for genetic diseases and cancer development. Conventional experimental approaches to study DNA damage required a researcher to choose between measuring bulk damage over the entire genome, with little or no resolution regarding a specific location, and obtaining data specific to a locus of interest, without a global perspective. Recent advances in high-throughput genomic tools overcame these limitations and provide high-resolution measurements simultaneously across the genome. In this review, we discuss the available methods for measuring DNA damage and their repair, focusing on genomewide assays for pyrimidine photodimers, the major types of damage induced by ultraviolet irradiation. These new genomic assays will be a powerful tool in identifying key components of genome stability and carcinogenesis.
| Original language | English |
|---|---|
| Pages (from-to) | 199-206 |
| Number of pages | 8 |
| Journal | Photochemistry and Photobiology |
| Volume | 93 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1 Jan 2017 |
Bibliographical note
Funding Information:We would like to thank Prof. Aziz Sancar for his mentorship, sharing the breadth of his knowledge, his scientific curiosity, his dedication to science, and his love and enthusiasm for discovery. J.H and S.A are supported by NIH grant GM118102.
Publisher Copyright:
© 2016 The American Society of Photobiology
