TY - JOUR
T1 - The chlorophyllases AtCLH1 and AtCLH2 are not essential for senescence-related chlorophyll breakdown in Arabidopsis thaliana
AU - Schenk, Nicole
AU - Schelbert, Silvia
AU - Kanwischer, Marion
AU - Goldschmidt, Eliezer E.
AU - Dörmann, Peter
AU - Hörtensteiner, Stefan
PY - 2007/11/27
Y1 - 2007/11/27
N2 - One important reaction of chlorophyll (chl) breakdown during plant senescence is the removal of the lipophilic phytol moiety by chlorophyllase. AtCLH1 and AtCLH2 were considered to be required for this reaction in Arabidopsis thaliana. Here we present evidence against this assumption. Using green fluorescent protein fusions, neither AtCLH isoform localizes to chloroplasts, the predicted site of chlorophyll breakdown. Furthermore, clh1 and clh2 single and double knockout lines are still able to degrade chlorophyll during senescence. From our data we conclude that AtCLHs are not required for senescence-related chlorophyll breakdown in vivo and propose that genuine chlorophyllase has not yet been molecularly identified.
AB - One important reaction of chlorophyll (chl) breakdown during plant senescence is the removal of the lipophilic phytol moiety by chlorophyllase. AtCLH1 and AtCLH2 were considered to be required for this reaction in Arabidopsis thaliana. Here we present evidence against this assumption. Using green fluorescent protein fusions, neither AtCLH isoform localizes to chloroplasts, the predicted site of chlorophyll breakdown. Furthermore, clh1 and clh2 single and double knockout lines are still able to degrade chlorophyll during senescence. From our data we conclude that AtCLHs are not required for senescence-related chlorophyll breakdown in vivo and propose that genuine chlorophyllase has not yet been molecularly identified.
KW - Chlorophyll breakdown
KW - Chlorophyll catabolites
KW - Chlorophyllase
KW - Phytol
KW - Senescence
UR - http://www.scopus.com/inward/record.url?scp=36048944783&partnerID=8YFLogxK
U2 - 10.1016/j.febslet.2007.10.060
DO - 10.1016/j.febslet.2007.10.060
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C2 - 17996203
AN - SCOPUS:36048944783
SN - 0014-5793
VL - 581
SP - 5517
EP - 5525
JO - FEBS Letters
JF - FEBS Letters
IS - 28
ER -