Abstract
Chronic myelogenous leukemia (CML) is a human disease associated with a consistent chromosomal translocation mat results in sequences from the c-abl locus on chromosome 9 being fused to sequences in a breakpoint chuter region (bcr) on chromosome 22. CML cells have two novel products: an 8.5-kilobase RNA transcript containing both abl and bcr and a 210-kilodalton phosphoprotein (P210) recognized by v-abl-specific antisera. To test whether the P210 is the product of the novel 8.5-kilobase bcr/abl fusion transcript, antibodies were prepared against c-abl and bcr determinants. By using these reagents and v-abl-specific antisera, it was demonstrated that the P210 in CML cells is indeed the protein product of the 8.5-kilobase transcript. By analogy to the gag/abl fusion protein of Abelson murine leukemia virus, the replacement of amino terminal c-abl sequences by bcr sequences in P210 may create a transforming protein involved in CML. A 190-kilodalton phosphoprotein that is a candidate for the normal bcr protein was identified in both HeLa and K562 cells.
Original language | English |
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Pages (from-to) | 212-214 |
Number of pages | 3 |
Journal | Science |
Volume | 233 |
Issue number | 4760 |
DOIs | |
State | Published - 1986 |
Externally published | Yes |