The chsA gene, encoding a class-I chitin synthase from Ampelomyces quisqualis

Nava Weiss; Abraham Sztejnberg; Oded Yarden

doi:10.1016/0378-1119(95)00722-9

The chsA gene, encoding a class-I chitin synthase from Ampelomyces quisqualis

Nava Weiss, Abraham Sztejnberg, Oded Yarden^*

^*Corresponding author for this work

Department of Plant Pathology and Microbiology

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Degenerate oligodeoxyribonucleotide primers, designed on the basis of conserved regions of the chitin synthase gene family, were used to amplify a fragment of the Ampelomyces quisqualis (Aq) chsA gene. Subsequently, the PCR product was used as a probe in order to identify and isolate genomic clones harboring the entire chsA gene. Aq chsA is 2786-nt long, has one intron and encodes a 910-amino-acid polypeptide belonging to the class-I chitin synthases. Low-stringency Southern hybridizations to Aq genomic DNA provided evidence for the presence of additional DNA fragments resembling chsA in the fungal genome, suggesting the presence of a multigene family of chitin synthases in Aq.

Original language	English
Pages (from-to)	99-102
Number of pages	4
Journal	Gene
Volume	168
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(95)00722-9
State	Published - 2 Feb 1996

Bibliographical note

Funding Information:
This work was supported in part by Ecogen Israel Partnership and by tbe Wolfson Research Awards administered by the Israel Academy of Sciences and Humanities.

Keywords

Biocontrol agent
Fungal cell wall
Hyperparasite
Powdery mildew

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0378-1119(95)00722-9

Cite this

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title = "The chsA gene, encoding a class-I chitin synthase from Ampelomyces quisqualis",

abstract = "Degenerate oligodeoxyribonucleotide primers, designed on the basis of conserved regions of the chitin synthase gene family, were used to amplify a fragment of the Ampelomyces quisqualis (Aq) chsA gene. Subsequently, the PCR product was used as a probe in order to identify and isolate genomic clones harboring the entire chsA gene. Aq chsA is 2786-nt long, has one intron and encodes a 910-amino-acid polypeptide belonging to the class-I chitin synthases. Low-stringency Southern hybridizations to Aq genomic DNA provided evidence for the presence of additional DNA fragments resembling chsA in the fungal genome, suggesting the presence of a multigene family of chitin synthases in Aq.",

keywords = "Biocontrol agent, Fungal cell wall, Hyperparasite, Powdery mildew",

author = "Nava Weiss and Abraham Sztejnberg and Oded Yarden",

note = "Funding Information: This work was supported in part by Ecogen Israel Partnership and by tbe Wolfson Research Awards administered by the Israel Academy of Sciences and Humanities.",

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AU - Yarden, Oded

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AB - Degenerate oligodeoxyribonucleotide primers, designed on the basis of conserved regions of the chitin synthase gene family, were used to amplify a fragment of the Ampelomyces quisqualis (Aq) chsA gene. Subsequently, the PCR product was used as a probe in order to identify and isolate genomic clones harboring the entire chsA gene. Aq chsA is 2786-nt long, has one intron and encodes a 910-amino-acid polypeptide belonging to the class-I chitin synthases. Low-stringency Southern hybridizations to Aq genomic DNA provided evidence for the presence of additional DNA fragments resembling chsA in the fungal genome, suggesting the presence of a multigene family of chitin synthases in Aq.

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The chsA gene, encoding a class-I chitin synthase from Ampelomyces quisqualis

Abstract

Bibliographical note

Keywords

UN SDGs

Access to Document

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