The chsA gene, encoding a class-I chitin synthase from Ampelomyces quisqualis

Nava Weiss, Abraham Sztejnberg, Oded Yarden*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Degenerate oligodeoxyribonucleotide primers, designed on the basis of conserved regions of the chitin synthase gene family, were used to amplify a fragment of the Ampelomyces quisqualis (Aq) chsA gene. Subsequently, the PCR product was used as a probe in order to identify and isolate genomic clones harboring the entire chsA gene. Aq chsA is 2786-nt long, has one intron and encodes a 910-amino-acid polypeptide belonging to the class-I chitin synthases. Low-stringency Southern hybridizations to Aq genomic DNA provided evidence for the presence of additional DNA fragments resembling chsA in the fungal genome, suggesting the presence of a multigene family of chitin synthases in Aq.

Original languageAmerican English
Pages (from-to)99-102
Number of pages4
JournalGene
Volume168
Issue number1
DOIs
StatePublished - 2 Feb 1996

Bibliographical note

Funding Information:
This work was supported in part by Ecogen Israel Partnership and by tbe Wolfson Research Awards administered by the Israel Academy of Sciences and Humanities.

Keywords

  • Biocontrol agent
  • Fungal cell wall
  • Hyperparasite
  • Powdery mildew

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