The contribution of storage medium and membranes in the microwave dielectric response of packed red blood cells suspension

Larisa Latypova; Gregory Barshtein; Dan Arbell; Yuri Feldman

doi:10.3390/app10051702

The contribution of storage medium and membranes in the microwave dielectric response of packed red blood cells suspension

Larisa Latypova, Gregory Barshtein^*, Dan Arbell, Yuri Feldman

^*Corresponding author for this work

Department of Biochemistry and Molecular Biology

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

During cold storage, packed red blood cells (PRBCs) undergo slow detrimental changes that are collectively termed storage lesion. The aging of the cells causes alterations in the composition of the storage-medium in the PRBC unit. In this paper, we present the comparison of the dielectric response of water in the primary (fresh) storage medium (citrate phosphate dextrose adenine solution, CPDA-1) versus the storage medium from three expired units of PRBCs. Dielectric response of the water molecules has been characterized by dielectric spectroscopy technique in the microwave frequency band (0.5-40 GHz). The dominant phenomenon is the significant increase of the dielectric strength and decrease the relaxation time τ for the samples of the stored medium in comparison with the fresh medium CPDA-1. Furthermore, we demonstrated that removing the ghosts from PRBC hemolysate did not cause the alteration of the dielectric spectrum of water. Thus, the contribution associated with water located near the cell membrane can be neglected in microwave dielectric measurements.

Original language	English
Article number	1702
Journal	Applied Sciences (Switzerland)
Volume	10
Issue number	5
DOIs	https://doi.org/10.3390/app10051702
State	Published - 1 Mar 2020

Bibliographical note

Funding Information:
This research was funded by The Swiss National Science Foundation CRSII5_180234 for the Research Project Premembrane and cytosolic water as a marker of red blood cell aging in vivo and in vitro and ISF 341/18. The role of water in the aging of Red Blood Cells. We would like to thank Keysight Technologies Israel Ltd. and INTERLLIGENT RF & Microwave Solutions for the loan of Vector Network Analyzer Agilent N5234B PNA-L. We would like to thank Anna Bogdanova for her support and scientific assistance.

Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

Broadening parameter α
CPDA-1
Cole-cole (CC) function
Dielectric strength ∆ε
Ghosts
Malondialdehyde (MDA) water main relaxation peak
Microwave dielectric spectroscopy (MDS)
Packed red blood cells (PRBC)
Red blood cells (RBC)
Relaxation time τ

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abstract = "During cold storage, packed red blood cells (PRBCs) undergo slow detrimental changes that are collectively termed storage lesion. The aging of the cells causes alterations in the composition of the storage-medium in the PRBC unit. In this paper, we present the comparison of the dielectric response of water in the primary (fresh) storage medium (citrate phosphate dextrose adenine solution, CPDA-1) versus the storage medium from three expired units of PRBCs. Dielectric response of the water molecules has been characterized by dielectric spectroscopy technique in the microwave frequency band (0.5-40 GHz). The dominant phenomenon is the significant increase of the dielectric strength and decrease the relaxation time τ for the samples of the stored medium in comparison with the fresh medium CPDA-1. Furthermore, we demonstrated that removing the ghosts from PRBC hemolysate did not cause the alteration of the dielectric spectrum of water. Thus, the contribution associated with water located near the cell membrane can be neglected in microwave dielectric measurements.",

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author = "Larisa Latypova and Gregory Barshtein and Dan Arbell and Yuri Feldman",

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AB - During cold storage, packed red blood cells (PRBCs) undergo slow detrimental changes that are collectively termed storage lesion. The aging of the cells causes alterations in the composition of the storage-medium in the PRBC unit. In this paper, we present the comparison of the dielectric response of water in the primary (fresh) storage medium (citrate phosphate dextrose adenine solution, CPDA-1) versus the storage medium from three expired units of PRBCs. Dielectric response of the water molecules has been characterized by dielectric spectroscopy technique in the microwave frequency band (0.5-40 GHz). The dominant phenomenon is the significant increase of the dielectric strength and decrease the relaxation time τ for the samples of the stored medium in comparison with the fresh medium CPDA-1. Furthermore, we demonstrated that removing the ghosts from PRBC hemolysate did not cause the alteration of the dielectric spectrum of water. Thus, the contribution associated with water located near the cell membrane can be neglected in microwave dielectric measurements.

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The contribution of storage medium and membranes in the microwave dielectric response of packed red blood cells suspension

Abstract

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Keywords

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