The design and evaluation of a novel targeted drug delivery system using cationic emulsion - Antibody conjugates

Danny Goldstein, Taher Nassar, Gregory Lambert, Jean Kadouche, Simon Benita*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

In an attempt to design a targeted drug delivery system to tumors' over-expressing H-ferritin specifically recognized by a monoclonal antibody, AMB8LK, a cationic emulsion - AMB8LK conjugate was prepared. A novel cross-linker molecule bearing maleimide group was synthesized and added to cationic emulsion formulation for AMB8LK Fab' fragment covalent coupling. NMR spectroscopy confirmed the cross-linker synthesis and the preservation of the active maleimide function. SDS gel-electrophoresis results corroborated the formation of the Fab' fragment. Different densities of Fab' fragments (10-200 Fab'/oil droplet) were conjugated to emulsion droplet interface and no changes in the physico-chemical properties were observed (∼120 nm size and zeta potential of ∼+ 30 mV). The coupling efficiency ranged from 55% to 70% and was visualized by TEM showing gold particles attached to the droplet interface. Cell culture studies demonstrated specific binding to cells as confirmed by the occurrence of the marked reduction in binding when free AMB8LK Mab was incubated before adding the AMB8LK-emulsion conjugate to the cells. The coupling of AMB8LK Fab' fragment to the cationic emulsion increased the cells uptake by 50% as compared to non-conjugated respective cationic emulsion. Appropriate conditions were, thus, identified for coupling AMB8LK Fab' fragment to cationic emulsion without altering the specificity and affinity of the Mab fragment to the tumor antigen.

Original languageAmerican English
Pages (from-to)418-432
Number of pages15
JournalJournal of Controlled Release
Volume108
Issue number2-3
DOIs
StatePublished - 28 Nov 2005

Bibliographical note

Funding Information:
This research was supported by THE ISRAEL SCIENCE FOUNDATION (grant no. 622/04).

Keywords

  • Antibody
  • Cancer
  • Cationic
  • Emulsion
  • Ferritin

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