The detection of heat-aggregated IgG (as a model for immune complexes) by reverse passive haemagglutination using a human monoclonal rheumatoid factor coupled to erythrocytes

C. J.R. Welsh; M. Steinitz; M. R. Clark; R. R.A. Coombs

doi:10.1016/0022-1759(87)90515-1

The detection of heat-aggregated IgG (as a model for immune complexes) by reverse passive haemagglutination using a human monoclonal rheumatoid factor coupled to erythrocytes

C. J.R. Welsh, M. Steinitz, M. R. Clark, R. R.A. Coombs^*

^*Corresponding author for this work

Institute for Medical Research Israel-Canada (IMRIC)

Research output: Contribution to journal › Article › peer-review

Abstract

A human monoclonal IgM rheumatoid factor (RF) produced in vitro by an Epstein-Barr virus (EBV)-immortalized cell line was purified by protein A-Sepharose adsorption and coupled by the chromic chloride method to human erythrocytes. The RF-coupled cells were incorporated in reverse passive haemagglutination (RPH) assays to detect immune complexes (IC) using heat-aggregated human IgG as a model system. The sensitivity of the RPH was comparable to an enzyme-linked immunosorbent assay (ELISA) using sheep C1q for the detection of ICs.

Original language	English
Pages (from-to)	271-274
Number of pages	4
Journal	Journal of Immunological Methods
Volume	104
Issue number	1-2
DOIs	https://doi.org/10.1016/0022-1759(87)90515-1
State	Published - 23 Nov 1987

Keywords

Immune complex assay
Monoclonal rheumatoid factor, human
Reverse passive hemagglutination

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10.1016/0022-1759(87)90515-1

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title = "The detection of heat-aggregated IgG (as a model for immune complexes) by reverse passive haemagglutination using a human monoclonal rheumatoid factor coupled to erythrocytes",

abstract = "A human monoclonal IgM rheumatoid factor (RF) produced in vitro by an Epstein-Barr virus (EBV)-immortalized cell line was purified by protein A-Sepharose adsorption and coupled by the chromic chloride method to human erythrocytes. The RF-coupled cells were incorporated in reverse passive haemagglutination (RPH) assays to detect immune complexes (IC) using heat-aggregated human IgG as a model system. The sensitivity of the RPH was comparable to an enzyme-linked immunosorbent assay (ELISA) using sheep C1q for the detection of ICs.",

keywords = "Immune complex assay, Monoclonal rheumatoid factor, human, Reverse passive hemagglutination",

author = "Welsh, {C. J.R.} and M. Steinitz and Clark, {M. R.} and Coombs, {R. R.A.}",

year = "1987",

month = nov,

day = "23",

doi = "10.1016/0022-1759(87)90515-1",

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The detection of heat-aggregated IgG (as a model for immune complexes) by reverse passive haemagglutination using a human monoclonal rheumatoid factor coupled to erythrocytes. / Welsh, C. J.R.; Steinitz, M.; Clark, M. R. et al.
In: Journal of Immunological Methods, Vol. 104, No. 1-2, 23.11.1987, p. 271-274.

Research output: Contribution to journal › Article › peer-review

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AU - Welsh, C. J.R.

AU - Steinitz, M.

AU - Clark, M. R.

AU - Coombs, R. R.A.

PY - 1987/11/23

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N2 - A human monoclonal IgM rheumatoid factor (RF) produced in vitro by an Epstein-Barr virus (EBV)-immortalized cell line was purified by protein A-Sepharose adsorption and coupled by the chromic chloride method to human erythrocytes. The RF-coupled cells were incorporated in reverse passive haemagglutination (RPH) assays to detect immune complexes (IC) using heat-aggregated human IgG as a model system. The sensitivity of the RPH was comparable to an enzyme-linked immunosorbent assay (ELISA) using sheep C1q for the detection of ICs.

AB - A human monoclonal IgM rheumatoid factor (RF) produced in vitro by an Epstein-Barr virus (EBV)-immortalized cell line was purified by protein A-Sepharose adsorption and coupled by the chromic chloride method to human erythrocytes. The RF-coupled cells were incorporated in reverse passive haemagglutination (RPH) assays to detect immune complexes (IC) using heat-aggregated human IgG as a model system. The sensitivity of the RPH was comparable to an enzyme-linked immunosorbent assay (ELISA) using sheep C1q for the detection of ICs.

KW - Immune complex assay

KW - Monoclonal rheumatoid factor, human

KW - Reverse passive hemagglutination

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The detection of heat-aggregated IgG (as a model for immune complexes) by reverse passive haemagglutination using a human monoclonal rheumatoid factor coupled to erythrocytes

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