The development of a loop-mediated isothermal amplification method (LAMP) for Echinococcus granulosis coprodetection

H. Salant; I. Abbasi; J. Hamburger

doi:10.4269/ajtmh.2012.12-0184

The development of a loop-mediated isothermal amplification method (LAMP) for Echinococcus granulosis coprodetection

H. Salant, I. Abbasi, J. Hamburger

Department of Microbiology and Molecular Genetics

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

We have previously developed a polymerase chain reaction (PCR) assay for detection of Echinococcus granulosus infection, which proved very sensitive and specific for identification of infected dogs. We have now developed a loop-mediated isothermal amplification (LAMP) assay, which amplifies the same genomic repeated sequences of E. granulosus for coprodetection. This assay enabled detection of a single egg in fecal samples and showed high species specificity for E. granulosus with no cross-amplification of DNA from closely related helminths, including Echinococcus multilocularis. Because the method does not require thermocycling for DNA amplification, or electrophoresis for amplicon detection, it can potentially be used for premortem identification of E. granulosus-infected dogs to enable large-scale surveys in endemic countries where highly specialized equipment to undertake PCR analysis is rare. Copyright © 2012 by The American Society of Tropical Medicine and Hygiene.

Original language	English
Pages (from-to)	883-887
Number of pages	5
Journal	American Journal of Tropical Medicine and Hygiene
Volume	87
Issue number	5
DOIs	https://doi.org/10.4269/ajtmh.2012.12-0184
State	Published - 2012

Bibliographical note

Cited By :36

Export Date: 30 January 2023

CODEN: AJTHA

Correspondence Address: Salant, H.; Department of Molecular Genetics, PO Box 12272, Jerusalem 91120, Israel; email: tsvis@ekmd.huji.ac.il

Chemicals/CAS: DNA, 9007-49-2; DNA polymerase, 37217-33-7; DNA Primers; DNA, Protozoan

Tradenames: Echinotest; NanoDrop-ND1000; PSP Spin Stool DNA Plus extraction Kit, invitek, Germany; Qiagen DNeasy Blood and Tissue extraction kit, Qiagen, United States

Manufacturers: invitek, Germany; Qiagen, United States

Funding details: DPR S92662F

Funding details: Office of Naval Research, ONR, DPR W19,493

Funding details: National Aeronautics and Space Administration, NASA, DPR W19,501

Funding text 1: NOAA provided flare and corrected GOES proton data. A. J. T. was supported by the ACE Guest Investigator Program (NASA DPR W19,501), the ISTP Solar Max Extended Science Program (NASA DPR S92662F), and the Office of Naval Research. W. F. D. was supported by the SEC Guest Investigator Program (NASA DPR W19,493).

References: Eckert, J., Gemmel, M.A., Meslin, F.X., Pawlowski, Z.S., (2001) WHO/OIE Manual on Echinococcosis in Humans and Animals: A Public Health Problem of Global Concern, pp. 1-263. , WHO, eds. Geneva: World Health Organization; Eckert, J., Conraths, F.J., Tackmann, K., Echinococcosis: An emerging or re-emerging zoonosis? (2000) Int J Parasitol, 30, pp. 1283-1294; Craig, P.S., Rogan, M.T., Allan, J.C., Detection, screening and community epidemiology of taeniid cestode zoonoses: Cystic echinococcosis, alveolar echinococcosis and neurocysticercosis (1996) Advances in Parasitology, 38, pp. 169-250; Allan, J.C., Craig, P.S., Garcia Novel, J., Mencos, F., Liu, D., Wang, Y., Wen, H., Zeyhle, E., Coproantigen detection for immunodiagnosis of echinococcosis and taeniasis in dogs and humans (1992) Parasitology, 104, pp. 347-356; Deplazes, P., Jimenez-Palacios, S., Gottstein, B., Skaggs, J., Eckert, J., Detection of Echinococcus coproantigens in stray dogs of northern Spain (1994) Appl Parasitol, 35, pp. 297-301; Malgor, R., Nonaka, N., Basmadjian, I., Sakai, H., Carambula, B., Oku, Y., Carmona, C., Kamiya, M., Coproantigen detectican in dogs experimentally and naturally infected with Echinococcus granulosus by a monoclonal antibody-based enzyme-linked immunosorbent assay (1997) International Journal for Parasitology, 27 (12), pp. 1605-1612. , DOI 10.1016/S0020-7519(97)00127-6, PII S0020751997001276; Moro, P.L., Bonifacio, N., Gilman, R.H., Lopera, L., Silva, B., Takumoto, R., Verastegui, M., Cabrera, L., Field diagnosis of Echinococcus granulosus infection among intermediate and definitive hosts in an endemic focus of human cystic echinococcosis (1999) Transactions of the Royal Society of Tropical Medicine and Hygiene, 93 (6), pp. 611-615; Casaravilla, C., Malgor, R., Rossi, A., Sakai, H., Nonaka, N., Kamiya, M., Carmona, C., Production and characterization of monoclonal antibodies against excretory/secretory products of adult Echinococcus granulosus, and their application to coproantigen detection (2005) Parasitology International, 54 (1), pp. 43-49. , DOI 10.1016/j.parint.2004.08.006; Stefanic, S., Shaikenov, B.S., Deplazes, P., Dinkel, A., Torgerson, P.R., Mathis, A., Polymerase chain reaction for detection of patent infections of Echinococcus granulosus ("sheep strain") in naturally infected dogs (2004) Parasitology Research, 92 (4), pp. 347-351. , DOI 10.1007/s00436-003-1043-y; Dinkel, A., Njoroge, E.M., Zimmermann, A., Walz, M., Zeyhle, E., Elmahdi, I.E., MacKenstedt, U., Romig, T., A PCR system for detection of species and genotypes of the Echinococcus granulosus-complex, with reference to the epidemiological situation in eastern Africa (2004) International Journal for Parasitology, 34 (5), pp. 645-653. , DOI 10.1016/j.ijpara.2003.12.013, PII S0020751904000116; Abbasi, I., Branzburg, A., Campos-Ponce, M., Abdel Hafez, S.K., Raoul, F., Craig, P.S., Hamburger, J., Coprodiagnosis of Echinococcus granulosus infection in dogs by amplification of a newly identified repeated DNA sequence (2003) Am J Trop Med Hyg, 69, pp. 324-330; Boufana, B.S., Campos-Ponce, M., Naidich, A., Buishi, I., Lahmar, S., Zeyhle, E., Jenkins, D.J., Craig, P.S., Evaluation of three PCR assays for the identification of the sheep strain (genotype 1) of Echinococcus granulosus in canid feces and parasite tissues (2008) American Journal of Tropical Medicine and Hygiene, 78 (5), pp. 777-783; Notomi, T., Okayama, H., Masubuchi, H., Yonekawa, T., Watanabe, K., Amino, N., Hase, T., Loop-mediated isothermal amplification of DNA (2000) Nucleic Acids Res, 28, pp. E63; Wastling, S.L., Picozzi, K., Kakembo, A.S., Welburn, S.C., LAMP for human African trypanosomiasis: A comparative study of detection formats (2010) PLoS Negl Trop Dis, 4, pp. E865; Iseki, H., Kawai, S., Takahashi, N., Hirai, M., Tanabe, K., Yokoyama, N., Igarashi, I., Evaluation of a loop-mediated isothermal amplification method as a tool for diagnosis of infection by the zoonotic simian malaria parasite Plasmodium knowlesi (2010) J Clin Microbiol, 48, pp. 2509-2514; Muller, H., Aysul, N., Liu, Z., Salih, D.A., Karagenc, T., Beyer, D., Kullmann, B., Seitzer, U., Development of a loop-mediated isothermal amplification (LAMP) assay for rapid diagnosis of Babesia canis infections (2010) Transbound Emerg Dis, 57, pp. 63-65; Aonuma, H., Yoshimura, A., Perera, N., Shinzawa, N., Bando, H., Oshiro, S., Nelson, B., Kanuka, H., Loop-mediated isothermal amplification applied to filarial parasites detection in the mosquito vectors: Dirofilaria immitis as a study model (2009) Parasit Vectors, 2, pp. 2-15; Abbasi, I., King, C.H., Muchiri, E.M., Hamburger, J., Detection of Schistosoma mansoni and Schistosoma haematobium DNA by loop-mediated isothermal amplification: Identification of infected snails from early prepatency (2010) Am J Trop Med Hyg, 83, pp. 427-432; Mathis, A., Deplazes, P., Copro-DNA tests for diagnosis of animal taeniid cestodes (2006) Parasitol Int, 55, pp. 587-590; Naidich, A., McManus, D.P., Canova, S.G., Gutierrez, A.M., Zhang, W., Guarnera, E.A., Rosenzvit, M.C., Patent and pre-patent detection of Echinococcus granulosus genotypes in the definitive host (2006) Mol Cell Probes, 20, pp. 1-10; Geojith, G., Dhanasekaran, S., Chandran, S.P., Kenneth, J., Efficacy of loop mediated isothermal amplification (LAMP) assay for the laboratory identification of Mycobacterium tuberculosis isolates in a resource limited setting (2011) J Microbiol Methods, 84, pp. 71-73

Keywords

DNA
DNA polymerase
parasite antigen
RNA 12S
agar gel electrophoresis
amplicon
animal experiment
animal model
article
autopsy
copro polymerase chain reaction
diagnostic kit
diagnostic test accuracy study
dog disease
echinococcosis
Echinococcus granulosus
Echinococcus multilocularis
egg
endemic disease
feces analysis
gene amplification
genome
gold standard
loop mediated isothermal amplification
nonhuman
parasite identification
polymerase chain reaction
sensitivity and specificity
species differentiation
spectrophotometry
tandem repeat
Animals
Base Sequence
DNA Primers
DNA, Protozoan
Feces
Humans
Nucleic Acid Amplification Techniques
Parasite Egg Count
Sensitivity and Specificity
Canis familiaris
Vermes

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10.4269/ajtmh.2012.12-0184

https://www.scopus.com/inward/record.uri?eid=2-s2.0-84869104317&doi=10.4269%2fajtmh.2012.12-0184&partnerID=40&md5=b1316deabb9c2048454e03fcb7078aa9

Cite this

@article{4890c9f0cafd493ba9d986b0763c16dd,

title = "The development of a loop-mediated isothermal amplification method (LAMP) for Echinococcus granulosis coprodetection",

abstract = "We have previously developed a polymerase chain reaction (PCR) assay for detection of Echinococcus granulosus infection, which proved very sensitive and specific for identification of infected dogs. We have now developed a loop-mediated isothermal amplification (LAMP) assay, which amplifies the same genomic repeated sequences of E. granulosus for coprodetection. This assay enabled detection of a single egg in fecal samples and showed high species specificity for E. granulosus with no cross-amplification of DNA from closely related helminths, including Echinococcus multilocularis. Because the method does not require thermocycling for DNA amplification, or electrophoresis for amplicon detection, it can potentially be used for premortem identification of E. granulosus-infected dogs to enable large-scale surveys in endemic countries where highly specialized equipment to undertake PCR analysis is rare. Copyright {\textcopyright} 2012 by The American Society of Tropical Medicine and Hygiene.",

keywords = "DNA, DNA polymerase, parasite antigen, RNA 12S, agar gel electrophoresis, amplicon, animal experiment, animal model, article, autopsy, copro polymerase chain reaction, diagnostic kit, diagnostic test accuracy study, dog disease, echinococcosis, Echinococcus granulosus, Echinococcus multilocularis, egg, endemic disease, feces analysis, gene amplification, genome, gold standard, loop mediated isothermal amplification, nonhuman, parasite identification, polymerase chain reaction, sensitivity and specificity, species differentiation, spectrophotometry, tandem repeat, Animals, Base Sequence, DNA Primers, DNA, Protozoan, Feces, Humans, Nucleic Acid Amplification Techniques, Parasite Egg Count, Sensitivity and Specificity, Canis familiaris, Vermes",

author = "H. Salant and I. Abbasi and J. Hamburger",

note = "Cited By :36 Export Date: 30 January 2023 CODEN: AJTHA Correspondence Address: Salant, H.; Department of Molecular Genetics, PO Box 12272, Jerusalem 91120, Israel; email: tsvis@ekmd.huji.ac.il Chemicals/CAS: DNA, 9007-49-2; DNA polymerase, 37217-33-7; DNA Primers; DNA, Protozoan Tradenames: Echinotest; NanoDrop-ND1000; PSP Spin Stool DNA Plus extraction Kit, invitek, Germany; Qiagen DNeasy Blood and Tissue extraction kit, Qiagen, United States Manufacturers: invitek, Germany; Qiagen, United States Funding details: DPR S92662F Funding details: Office of Naval Research, ONR, DPR W19,493 Funding details: National Aeronautics and Space Administration, NASA, DPR W19,501 Funding text 1: NOAA provided flare and corrected GOES proton data. A. J. T. was supported by the ACE Guest Investigator Program (NASA DPR W19,501), the ISTP Solar Max Extended Science Program (NASA DPR S92662F), and the Office of Naval Research. W. F. D. was supported by the SEC Guest Investigator Program (NASA DPR W19,493). References: Eckert, J., Gemmel, M.A., Meslin, F.X., Pawlowski, Z.S., (2001) WHO/OIE Manual on Echinococcosis in Humans and Animals: A Public Health Problem of Global Concern, pp. 1-263. , WHO, eds. Geneva: World Health Organization; Eckert, J., Conraths, F.J., Tackmann, K., Echinococcosis: An emerging or re-emerging zoonosis? (2000) Int J Parasitol, 30, pp. 1283-1294; Craig, P.S., Rogan, M.T., Allan, J.C., Detection, screening and community epidemiology of taeniid cestode zoonoses: Cystic echinococcosis, alveolar echinococcosis and neurocysticercosis (1996) Advances in Parasitology, 38, pp. 169-250; Allan, J.C., Craig, P.S., Garcia Novel, J., Mencos, F., Liu, D., Wang, Y., Wen, H., Zeyhle, E., Coproantigen detection for immunodiagnosis of echinococcosis and taeniasis in dogs and humans (1992) Parasitology, 104, pp. 347-356; Deplazes, P., Jimenez-Palacios, S., Gottstein, B., Skaggs, J., Eckert, J., Detection of Echinococcus coproantigens in stray dogs of northern Spain (1994) Appl Parasitol, 35, pp. 297-301; Malgor, R., Nonaka, N., Basmadjian, I., Sakai, H., Carambula, B., Oku, Y., Carmona, C., Kamiya, M., Coproantigen detectican in dogs experimentally and naturally infected with Echinococcus granulosus by a monoclonal antibody-based enzyme-linked immunosorbent assay (1997) International Journal for Parasitology, 27 (12), pp. 1605-1612. , DOI 10.1016/S0020-7519(97)00127-6, PII S0020751997001276; Moro, P.L., Bonifacio, N., Gilman, R.H., Lopera, L., Silva, B., Takumoto, R., Verastegui, M., Cabrera, L., Field diagnosis of Echinococcus granulosus infection among intermediate and definitive hosts in an endemic focus of human cystic echinococcosis (1999) Transactions of the Royal Society of Tropical Medicine and Hygiene, 93 (6), pp. 611-615; Casaravilla, C., Malgor, R., Rossi, A., Sakai, H., Nonaka, N., Kamiya, M., Carmona, C., Production and characterization of monoclonal antibodies against excretory/secretory products of adult Echinococcus granulosus, and their application to coproantigen detection (2005) Parasitology International, 54 (1), pp. 43-49. , DOI 10.1016/j.parint.2004.08.006; Stefanic, S., Shaikenov, B.S., Deplazes, P., Dinkel, A., Torgerson, P.R., Mathis, A., Polymerase chain reaction for detection of patent infections of Echinococcus granulosus ({"}sheep strain{"}) in naturally infected dogs (2004) Parasitology Research, 92 (4), pp. 347-351. , DOI 10.1007/s00436-003-1043-y; Dinkel, A., Njoroge, E.M., Zimmermann, A., Walz, M., Zeyhle, E., Elmahdi, I.E., MacKenstedt, U., Romig, T., A PCR system for detection of species and genotypes of the Echinococcus granulosus-complex, with reference to the epidemiological situation in eastern Africa (2004) International Journal for Parasitology, 34 (5), pp. 645-653. , DOI 10.1016/j.ijpara.2003.12.013, PII S0020751904000116; Abbasi, I., Branzburg, A., Campos-Ponce, M., Abdel Hafez, S.K., Raoul, F., Craig, P.S., Hamburger, J., Coprodiagnosis of Echinococcus granulosus infection in dogs by amplification of a newly identified repeated DNA sequence (2003) Am J Trop Med Hyg, 69, pp. 324-330; Boufana, B.S., Campos-Ponce, M., Naidich, A., Buishi, I., Lahmar, S., Zeyhle, E., Jenkins, D.J., Craig, P.S., Evaluation of three PCR assays for the identification of the sheep strain (genotype 1) of Echinococcus granulosus in canid feces and parasite tissues (2008) American Journal of Tropical Medicine and Hygiene, 78 (5), pp. 777-783; Notomi, T., Okayama, H., Masubuchi, H., Yonekawa, T., Watanabe, K., Amino, N., Hase, T., Loop-mediated isothermal amplification of DNA (2000) Nucleic Acids Res, 28, pp. E63; Wastling, S.L., Picozzi, K., Kakembo, A.S., Welburn, S.C., LAMP for human African trypanosomiasis: A comparative study of detection formats (2010) PLoS Negl Trop Dis, 4, pp. E865; Iseki, H., Kawai, S., Takahashi, N., Hirai, M., Tanabe, K., Yokoyama, N., Igarashi, I., Evaluation of a loop-mediated isothermal amplification method as a tool for diagnosis of infection by the zoonotic simian malaria parasite Plasmodium knowlesi (2010) J Clin Microbiol, 48, pp. 2509-2514; Muller, H., Aysul, N., Liu, Z., Salih, D.A., Karagenc, T., Beyer, D., Kullmann, B., Seitzer, U., Development of a loop-mediated isothermal amplification (LAMP) assay for rapid diagnosis of Babesia canis infections (2010) Transbound Emerg Dis, 57, pp. 63-65; Aonuma, H., Yoshimura, A., Perera, N., Shinzawa, N., Bando, H., Oshiro, S., Nelson, B., Kanuka, H., Loop-mediated isothermal amplification applied to filarial parasites detection in the mosquito vectors: Dirofilaria immitis as a study model (2009) Parasit Vectors, 2, pp. 2-15; Abbasi, I., King, C.H., Muchiri, E.M., Hamburger, J., Detection of Schistosoma mansoni and Schistosoma haematobium DNA by loop-mediated isothermal amplification: Identification of infected snails from early prepatency (2010) Am J Trop Med Hyg, 83, pp. 427-432; Mathis, A., Deplazes, P., Copro-DNA tests for diagnosis of animal taeniid cestodes (2006) Parasitol Int, 55, pp. 587-590; Naidich, A., McManus, D.P., Canova, S.G., Gutierrez, A.M., Zhang, W., Guarnera, E.A., Rosenzvit, M.C., Patent and pre-patent detection of Echinococcus granulosus genotypes in the definitive host (2006) Mol Cell Probes, 20, pp. 1-10; Geojith, G., Dhanasekaran, S., Chandran, S.P., Kenneth, J., Efficacy of loop mediated isothermal amplification (LAMP) assay for the laboratory identification of Mycobacterium tuberculosis isolates in a resource limited setting (2011) J Microbiol Methods, 84, pp. 71-73",

year = "2012",

doi = "10.4269/ajtmh.2012.12-0184",

language = "אנגלית",

volume = "87",

pages = "883--887",

journal = "American Journal of Tropical Medicine and Hygiene",

issn = "0002-9637",

publisher = "American Society of Tropical Medicine and Hygiene",

number = "5",

}

TY - JOUR

T1 - The development of a loop-mediated isothermal amplification method (LAMP) for Echinococcus granulosis coprodetection

AU - Salant, H.

AU - Abbasi, I.

AU - Hamburger, J.

N1 - Cited By :36 Export Date: 30 January 2023 CODEN: AJTHA Correspondence Address: Salant, H.; Department of Molecular Genetics, PO Box 12272, Jerusalem 91120, Israel; email: tsvis@ekmd.huji.ac.il Chemicals/CAS: DNA, 9007-49-2; DNA polymerase, 37217-33-7; DNA Primers; DNA, Protozoan Tradenames: Echinotest; NanoDrop-ND1000; PSP Spin Stool DNA Plus extraction Kit, invitek, Germany; Qiagen DNeasy Blood and Tissue extraction kit, Qiagen, United States Manufacturers: invitek, Germany; Qiagen, United States Funding details: DPR S92662F Funding details: Office of Naval Research, ONR, DPR W19,493 Funding details: National Aeronautics and Space Administration, NASA, DPR W19,501 Funding text 1: NOAA provided flare and corrected GOES proton data. A. J. T. was supported by the ACE Guest Investigator Program (NASA DPR W19,501), the ISTP Solar Max Extended Science Program (NASA DPR S92662F), and the Office of Naval Research. W. F. D. was supported by the SEC Guest Investigator Program (NASA DPR W19,493). References: Eckert, J., Gemmel, M.A., Meslin, F.X., Pawlowski, Z.S., (2001) WHO/OIE Manual on Echinococcosis in Humans and Animals: A Public Health Problem of Global Concern, pp. 1-263. , WHO, eds. Geneva: World Health Organization; Eckert, J., Conraths, F.J., Tackmann, K., Echinococcosis: An emerging or re-emerging zoonosis? (2000) Int J Parasitol, 30, pp. 1283-1294; Craig, P.S., Rogan, M.T., Allan, J.C., Detection, screening and community epidemiology of taeniid cestode zoonoses: Cystic echinococcosis, alveolar echinococcosis and neurocysticercosis (1996) Advances in Parasitology, 38, pp. 169-250; Allan, J.C., Craig, P.S., Garcia Novel, J., Mencos, F., Liu, D., Wang, Y., Wen, H., Zeyhle, E., Coproantigen detection for immunodiagnosis of echinococcosis and taeniasis in dogs and humans (1992) Parasitology, 104, pp. 347-356; Deplazes, P., Jimenez-Palacios, S., Gottstein, B., Skaggs, J., Eckert, J., Detection of Echinococcus coproantigens in stray dogs of northern Spain (1994) Appl Parasitol, 35, pp. 297-301; Malgor, R., Nonaka, N., Basmadjian, I., Sakai, H., Carambula, B., Oku, Y., Carmona, C., Kamiya, M., Coproantigen detectican in dogs experimentally and naturally infected with Echinococcus granulosus by a monoclonal antibody-based enzyme-linked immunosorbent assay (1997) International Journal for Parasitology, 27 (12), pp. 1605-1612. , DOI 10.1016/S0020-7519(97)00127-6, PII S0020751997001276; Moro, P.L., Bonifacio, N., Gilman, R.H., Lopera, L., Silva, B., Takumoto, R., Verastegui, M., Cabrera, L., Field diagnosis of Echinococcus granulosus infection among intermediate and definitive hosts in an endemic focus of human cystic echinococcosis (1999) Transactions of the Royal Society of Tropical Medicine and Hygiene, 93 (6), pp. 611-615; Casaravilla, C., Malgor, R., Rossi, A., Sakai, H., Nonaka, N., Kamiya, M., Carmona, C., Production and characterization of monoclonal antibodies against excretory/secretory products of adult Echinococcus granulosus, and their application to coproantigen detection (2005) Parasitology International, 54 (1), pp. 43-49. , DOI 10.1016/j.parint.2004.08.006; Stefanic, S., Shaikenov, B.S., Deplazes, P., Dinkel, A., Torgerson, P.R., Mathis, A., Polymerase chain reaction for detection of patent infections of Echinococcus granulosus ("sheep strain") in naturally infected dogs (2004) Parasitology Research, 92 (4), pp. 347-351. , DOI 10.1007/s00436-003-1043-y; Dinkel, A., Njoroge, E.M., Zimmermann, A., Walz, M., Zeyhle, E., Elmahdi, I.E., MacKenstedt, U., Romig, T., A PCR system for detection of species and genotypes of the Echinococcus granulosus-complex, with reference to the epidemiological situation in eastern Africa (2004) International Journal for Parasitology, 34 (5), pp. 645-653. , DOI 10.1016/j.ijpara.2003.12.013, PII S0020751904000116; Abbasi, I., Branzburg, A., Campos-Ponce, M., Abdel Hafez, S.K., Raoul, F., Craig, P.S., Hamburger, J., Coprodiagnosis of Echinococcus granulosus infection in dogs by amplification of a newly identified repeated DNA sequence (2003) Am J Trop Med Hyg, 69, pp. 324-330; Boufana, B.S., Campos-Ponce, M., Naidich, A., Buishi, I., Lahmar, S., Zeyhle, E., Jenkins, D.J., Craig, P.S., Evaluation of three PCR assays for the identification of the sheep strain (genotype 1) of Echinococcus granulosus in canid feces and parasite tissues (2008) American Journal of Tropical Medicine and Hygiene, 78 (5), pp. 777-783; Notomi, T., Okayama, H., Masubuchi, H., Yonekawa, T., Watanabe, K., Amino, N., Hase, T., Loop-mediated isothermal amplification of DNA (2000) Nucleic Acids Res, 28, pp. E63; Wastling, S.L., Picozzi, K., Kakembo, A.S., Welburn, S.C., LAMP for human African trypanosomiasis: A comparative study of detection formats (2010) PLoS Negl Trop Dis, 4, pp. E865; Iseki, H., Kawai, S., Takahashi, N., Hirai, M., Tanabe, K., Yokoyama, N., Igarashi, I., Evaluation of a loop-mediated isothermal amplification method as a tool for diagnosis of infection by the zoonotic simian malaria parasite Plasmodium knowlesi (2010) J Clin Microbiol, 48, pp. 2509-2514; Muller, H., Aysul, N., Liu, Z., Salih, D.A., Karagenc, T., Beyer, D., Kullmann, B., Seitzer, U., Development of a loop-mediated isothermal amplification (LAMP) assay for rapid diagnosis of Babesia canis infections (2010) Transbound Emerg Dis, 57, pp. 63-65; Aonuma, H., Yoshimura, A., Perera, N., Shinzawa, N., Bando, H., Oshiro, S., Nelson, B., Kanuka, H., Loop-mediated isothermal amplification applied to filarial parasites detection in the mosquito vectors: Dirofilaria immitis as a study model (2009) Parasit Vectors, 2, pp. 2-15; Abbasi, I., King, C.H., Muchiri, E.M., Hamburger, J., Detection of Schistosoma mansoni and Schistosoma haematobium DNA by loop-mediated isothermal amplification: Identification of infected snails from early prepatency (2010) Am J Trop Med Hyg, 83, pp. 427-432; Mathis, A., Deplazes, P., Copro-DNA tests for diagnosis of animal taeniid cestodes (2006) Parasitol Int, 55, pp. 587-590; Naidich, A., McManus, D.P., Canova, S.G., Gutierrez, A.M., Zhang, W., Guarnera, E.A., Rosenzvit, M.C., Patent and pre-patent detection of Echinococcus granulosus genotypes in the definitive host (2006) Mol Cell Probes, 20, pp. 1-10; Geojith, G., Dhanasekaran, S., Chandran, S.P., Kenneth, J., Efficacy of loop mediated isothermal amplification (LAMP) assay for the laboratory identification of Mycobacterium tuberculosis isolates in a resource limited setting (2011) J Microbiol Methods, 84, pp. 71-73

PY - 2012

Y1 - 2012

N2 - We have previously developed a polymerase chain reaction (PCR) assay for detection of Echinococcus granulosus infection, which proved very sensitive and specific for identification of infected dogs. We have now developed a loop-mediated isothermal amplification (LAMP) assay, which amplifies the same genomic repeated sequences of E. granulosus for coprodetection. This assay enabled detection of a single egg in fecal samples and showed high species specificity for E. granulosus with no cross-amplification of DNA from closely related helminths, including Echinococcus multilocularis. Because the method does not require thermocycling for DNA amplification, or electrophoresis for amplicon detection, it can potentially be used for premortem identification of E. granulosus-infected dogs to enable large-scale surveys in endemic countries where highly specialized equipment to undertake PCR analysis is rare. Copyright © 2012 by The American Society of Tropical Medicine and Hygiene.

AB - We have previously developed a polymerase chain reaction (PCR) assay for detection of Echinococcus granulosus infection, which proved very sensitive and specific for identification of infected dogs. We have now developed a loop-mediated isothermal amplification (LAMP) assay, which amplifies the same genomic repeated sequences of E. granulosus for coprodetection. This assay enabled detection of a single egg in fecal samples and showed high species specificity for E. granulosus with no cross-amplification of DNA from closely related helminths, including Echinococcus multilocularis. Because the method does not require thermocycling for DNA amplification, or electrophoresis for amplicon detection, it can potentially be used for premortem identification of E. granulosus-infected dogs to enable large-scale surveys in endemic countries where highly specialized equipment to undertake PCR analysis is rare. Copyright © 2012 by The American Society of Tropical Medicine and Hygiene.

KW - DNA

KW - DNA polymerase

KW - parasite antigen

KW - RNA 12S

KW - agar gel electrophoresis

KW - amplicon

KW - animal experiment

KW - animal model

KW - article

KW - autopsy

KW - copro polymerase chain reaction

KW - diagnostic kit

KW - diagnostic test accuracy study

KW - dog disease

KW - echinococcosis

KW - Echinococcus granulosus

KW - Echinococcus multilocularis

KW - egg

KW - endemic disease

KW - feces analysis

KW - gene amplification

KW - genome

KW - gold standard

KW - loop mediated isothermal amplification

KW - nonhuman

KW - parasite identification

KW - polymerase chain reaction

KW - sensitivity and specificity

KW - species differentiation

KW - spectrophotometry

KW - tandem repeat

KW - Animals

KW - Base Sequence

KW - DNA Primers

KW - DNA, Protozoan

KW - Feces

KW - Humans

KW - Nucleic Acid Amplification Techniques

KW - Parasite Egg Count

KW - Sensitivity and Specificity

KW - Canis familiaris

KW - Vermes

UR - http://www.scopus.com/inward/record.url?scp=84869104317&partnerID=8YFLogxK

U2 - 10.4269/ajtmh.2012.12-0184

DO - 10.4269/ajtmh.2012.12-0184

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

SN - 0002-9637

VL - 87

SP - 883

EP - 887

JO - American Journal of Tropical Medicine and Hygiene

JF - American Journal of Tropical Medicine and Hygiene

IS - 5

ER -

The development of a loop-mediated isothermal amplification method (LAMP) for Echinococcus granulosis coprodetection

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