The development of a loop-mediated isothermal amplification method (LAMP) for Echinococcus granulosis coprodetection

H. Salant, I. Abbasi, J. Hamburger

Research output: Contribution to journalArticlepeer-review

38 Scopus citations


We have previously developed a polymerase chain reaction (PCR) assay for detection of Echinococcus granulosus infection, which proved very sensitive and specific for identification of infected dogs. We have now developed a loop-mediated isothermal amplification (LAMP) assay, which amplifies the same genomic repeated sequences of E. granulosus for coprodetection. This assay enabled detection of a single egg in fecal samples and showed high species specificity for E. granulosus with no cross-amplification of DNA from closely related helminths, including Echinococcus multilocularis. Because the method does not require thermocycling for DNA amplification, or electrophoresis for amplicon detection, it can potentially be used for premortem identification of E. granulosus-infected dogs to enable large-scale surveys in endemic countries where highly specialized equipment to undertake PCR analysis is rare. Copyright © 2012 by The American Society of Tropical Medicine and Hygiene.
Original languageEnglish
Pages (from-to)883-887
Number of pages5
JournalAmerican Journal of Tropical Medicine and Hygiene
Issue number5
StatePublished - 2012

Bibliographical note

Cited By :36

Export Date: 30 January 2023


Correspondence Address: Salant, H.; Department of Molecular Genetics, PO Box 12272, Jerusalem 91120, Israel; email:

Chemicals/CAS: DNA, 9007-49-2; DNA polymerase, 37217-33-7; DNA Primers; DNA, Protozoan

Tradenames: Echinotest; NanoDrop-ND1000; PSP Spin Stool DNA Plus extraction Kit, invitek, Germany; Qiagen DNeasy Blood and Tissue extraction kit, Qiagen, United States

Manufacturers: invitek, Germany; Qiagen, United States

Funding details: DPR S92662F

Funding details: Office of Naval Research, ONR, DPR W19,493

Funding details: National Aeronautics and Space Administration, NASA, DPR W19,501

Funding text 1: NOAA provided flare and corrected GOES proton data. A. J. T. was supported by the ACE Guest Investigator Program (NASA DPR W19,501), the ISTP Solar Max Extended Science Program (NASA DPR S92662F), and the Office of Naval Research. W. F. D. was supported by the SEC Guest Investigator Program (NASA DPR W19,493).

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  • DNA
  • DNA polymerase
  • parasite antigen
  • RNA 12S
  • agar gel electrophoresis
  • amplicon
  • animal experiment
  • animal model
  • article
  • autopsy
  • copro polymerase chain reaction
  • diagnostic kit
  • diagnostic test accuracy study
  • dog disease
  • echinococcosis
  • Echinococcus granulosus
  • Echinococcus multilocularis
  • egg
  • endemic disease
  • feces analysis
  • gene amplification
  • genome
  • gold standard
  • loop mediated isothermal amplification
  • nonhuman
  • parasite identification
  • polymerase chain reaction
  • sensitivity and specificity
  • species differentiation
  • spectrophotometry
  • tandem repeat
  • Animals
  • Base Sequence
  • DNA Primers
  • DNA, Protozoan
  • Feces
  • Humans
  • Nucleic Acid Amplification Techniques
  • Parasite Egg Count
  • Sensitivity and Specificity
  • Canis familiaris
  • Vermes


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