The development of a loop-mediated isothermal amplification method (LAMP) for Echinococcus granulosis coprodetection

Harold Salant*, Ibrahim Abbasi, Joseph Hamburger

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

We have previously developed a polymerase chain reaction (PCR) assay for detection of Echinococcus granulosus infection, which proved very sensitive and specific for identification of infected dogs. We have now developed a loop-mediated isothermal amplification (LAMP) assay, which amplifies the same genomic repeated sequences of E. granulosus for coprodetection. This assay enabled detection of a single egg in fecal samples and showed high species specificity for E. granulosus with no cross-amplification of DNA from closely related helminths, including Echinococcus multilocularis. Because the method does not require thermocycling for DNA amplification, or electrophoresis for amplicon detection, it can potentially be used for premortem identification of E. granulosus-infected dogs to enable large-scale surveys in endemic countries where highly specialized equipment to undertake PCR analysis is rare.

Original languageAmerican English
Pages (from-to)883-887
Number of pages5
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume87
Issue number5
DOIs
StatePublished - Nov 2012

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