The dorsalizing and neural inducing gene follistatin is an antagonist of BMP-4

Abraham Fainsod; Kirsten Deißler; Ronit Yelin; Karen Marom; Michal Epstein; Graciela Pillemer; Herbert Steinbeisser; Martin Blum

doi:10.1016/S0925-4773(97)00673-4

The dorsalizing and neural inducing gene follistatin is an antagonist of BMP-4

Abraham Fainsod^*, Kirsten Deißler, Ronit Yelin, Karen Marom, Michal Epstein, Graciela Pillemer, Herbert Steinbeisser, Martin Blum

^*Corresponding author for this work

Department of Developmental Biology and Cancer Research

Research output: Contribution to journal › Article › peer-review

317 Scopus citations

Abstract

Specific signaling molecules play a pivotal role in the induction and specification of tissues during early vertebrate embryogenesis. BMP-4 specifies ventral mesoderm differentiation and inhibits neural induction in Xenopus,whereas three molecules secreted from the organizer, noggin, follistatin and chordin dorsalize mesoderm and promote neural induction. Here we report that follistatin antagonizes the activities of BMP-4 in frog embryos and mouse teratocarcinoma cells. In Xenopus embryos follistatin blocks the ventralizing effect of BMP-4. In mouse P19 cells follistatin promotes neural differentiation. BMP-4 antagonizes the action of follistatin and prevents neural differentiation. In addition we show that the follistatin and BMP-4 proteins can interact directly in vitro. These data provide evidence that follistatin might play a role in modulating BMP-4 activity in vivo.

Original language	English
Pages (from-to)	39-50
Number of pages	12
Journal	Mechanisms of Development
Volume	63
Issue number	1
DOIs	https://doi.org/10.1016/S0925-4773(97)00673-4
State	Published - Apr 1997

Bibliographical note

Funding Information:
We thank E. De Robertis and Y. Sasai for the follistatin clone, D. Melton for the BMP receptor clones, K. Masuhara for the BMP-4 antibody and Genetics Institute for recombinant human BMP-4 protein. We also thank J. Yisraeli and E. Shapira for advice. This work was supported in part by the Council for Tobacco Research, USA to A.F.

Keywords

BMP-4
Embryonic development
Follistatin
Mouse
Spemann's organizer
Xenopus

Access to Document

10.1016/S0925-4773(97)00673-4

Cite this

@article{4a8029bfdd59420d8dcb3e484ca365a6,

title = "The dorsalizing and neural inducing gene follistatin is an antagonist of BMP-4",

abstract = "Specific signaling molecules play a pivotal role in the induction and specification of tissues during early vertebrate embryogenesis. BMP-4 specifies ventral mesoderm differentiation and inhibits neural induction in Xenopus,whereas three molecules secreted from the organizer, noggin, follistatin and chordin dorsalize mesoderm and promote neural induction. Here we report that follistatin antagonizes the activities of BMP-4 in frog embryos and mouse teratocarcinoma cells. In Xenopus embryos follistatin blocks the ventralizing effect of BMP-4. In mouse P19 cells follistatin promotes neural differentiation. BMP-4 antagonizes the action of follistatin and prevents neural differentiation. In addition we show that the follistatin and BMP-4 proteins can interact directly in vitro. These data provide evidence that follistatin might play a role in modulating BMP-4 activity in vivo.",

keywords = "BMP-4, Embryonic development, Follistatin, Mouse, Spemann's organizer, Xenopus",

author = "Abraham Fainsod and Kirsten Dei{\ss}ler and Ronit Yelin and Karen Marom and Michal Epstein and Graciela Pillemer and Herbert Steinbeisser and Martin Blum",

note = "Funding Information: We thank E. De Robertis and Y. Sasai for the follistatin clone, D. Melton for the BMP receptor clones, K. Masuhara for the BMP-4 antibody and Genetics Institute for recombinant human BMP-4 protein. We also thank J. Yisraeli and E. Shapira for advice. This work was supported in part by the Council for Tobacco Research, USA to A.F.",

year = "1997",

month = apr,

doi = "10.1016/S0925-4773(97)00673-4",

language = "אנגלית",

volume = "63",

pages = "39--50",

journal = "Mechanisms of Development",

issn = "0925-4773",

publisher = "Elsevier B.V.",

number = "1",

}

TY - JOUR

T1 - The dorsalizing and neural inducing gene follistatin is an antagonist of BMP-4

AU - Fainsod, Abraham

AU - Deißler, Kirsten

AU - Yelin, Ronit

AU - Marom, Karen

AU - Epstein, Michal

AU - Pillemer, Graciela

AU - Steinbeisser, Herbert

AU - Blum, Martin

N1 - Funding Information: We thank E. De Robertis and Y. Sasai for the follistatin clone, D. Melton for the BMP receptor clones, K. Masuhara for the BMP-4 antibody and Genetics Institute for recombinant human BMP-4 protein. We also thank J. Yisraeli and E. Shapira for advice. This work was supported in part by the Council for Tobacco Research, USA to A.F.

PY - 1997/4

Y1 - 1997/4

N2 - Specific signaling molecules play a pivotal role in the induction and specification of tissues during early vertebrate embryogenesis. BMP-4 specifies ventral mesoderm differentiation and inhibits neural induction in Xenopus,whereas three molecules secreted from the organizer, noggin, follistatin and chordin dorsalize mesoderm and promote neural induction. Here we report that follistatin antagonizes the activities of BMP-4 in frog embryos and mouse teratocarcinoma cells. In Xenopus embryos follistatin blocks the ventralizing effect of BMP-4. In mouse P19 cells follistatin promotes neural differentiation. BMP-4 antagonizes the action of follistatin and prevents neural differentiation. In addition we show that the follistatin and BMP-4 proteins can interact directly in vitro. These data provide evidence that follistatin might play a role in modulating BMP-4 activity in vivo.

AB - Specific signaling molecules play a pivotal role in the induction and specification of tissues during early vertebrate embryogenesis. BMP-4 specifies ventral mesoderm differentiation and inhibits neural induction in Xenopus,whereas three molecules secreted from the organizer, noggin, follistatin and chordin dorsalize mesoderm and promote neural induction. Here we report that follistatin antagonizes the activities of BMP-4 in frog embryos and mouse teratocarcinoma cells. In Xenopus embryos follistatin blocks the ventralizing effect of BMP-4. In mouse P19 cells follistatin promotes neural differentiation. BMP-4 antagonizes the action of follistatin and prevents neural differentiation. In addition we show that the follistatin and BMP-4 proteins can interact directly in vitro. These data provide evidence that follistatin might play a role in modulating BMP-4 activity in vivo.

KW - BMP-4

KW - Embryonic development

KW - Follistatin

KW - Mouse

KW - Spemann's organizer

KW - Xenopus

UR - http://www.scopus.com/inward/record.url?scp=0342684469&partnerID=8YFLogxK

U2 - 10.1016/S0925-4773(97)00673-4

DO - 10.1016/S0925-4773(97)00673-4

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 9178255

AN - SCOPUS:0342684469

SN - 0925-4773

VL - 63

SP - 39

EP - 50

JO - Mechanisms of Development

JF - Mechanisms of Development

IS - 1

ER -

The dorsalizing and neural inducing gene follistatin is an antagonist of BMP-4

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Cite this