The dps promoter is activated by OxyR during growth and by IHF and σs in stationary phase

Shoshy Altuvia, Marta Almiron, Gjalt Huisman, Roberto Kolter, Gisela Storz*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

368 Scopus citations

Abstract

Dps is a non‐specific DNA‐binding protein abundant In starved Escherichia coli cells and is important for the defence against hydrogen peroxide. We found that dps mRNA levels are controlled by rpoS‐encoded σs, the transcriptional activator OxyR and the histone‐like IHF protein, in exponentially growing cells, dps is induced by treatment with hydrogen peroxide in an OxyR‐dependent manner. This OxyR‐dependent induction occurs only during log phase, although the OxyR protein is present in stationary phase, in the stationary phase cells, dps is expressed in a σs ‐ and IHF‐dependent manner. The purified OxyR and IHF proteins are also shown to bind upstream of the dps promoter. Our results suggest that the dps promoter is recognized by both σ70‐holoenzyme and σs‐holoenzyme, since OxyR acts through σ70 and the starts of the OxyR‐ and σs‐dependent transcripts are identical.

Original languageAmerican English
Pages (from-to)265-272
Number of pages8
JournalMolecular Microbiology
Volume13
Issue number2
DOIs
StatePublished - Jul 1994
Externally publishedYes

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