TY - JOUR
T1 - The effect of bezafibrate and long-chain fatty acids on peroxisomal activities in cultured rat hepatocytes
AU - Hertz, Rachel
AU - Arnon, Judith
AU - Bar-Tana, Jacob
PY - 1985/9/11
Y1 - 1985/9/11
N2 - Peroxisomal activities have been evaluated in cultured rat hepatocytes in the presence of bezafibrate or long-chain fatty acids added to the culture medium. All activities decreased continuously over a time period of 100 h in culture but selected activities were relatively increased as a function of the added effectors. This relative increase in peroxisomal activities was dose-dependent, discernible within the first 24 h in culture and consisted of activities related specifically to peroxisomal fatty acyl β-oxidation, e.g., cyanide-insensitive palmitoyl-CoA oxidation, H2O2-forming palmitoyl-CoA oxidase and heat-labile enoyl-CoA hydratase. Peroxisomal catalase or mitochondrial fatty acyl β-oxidation (cyanide-sensitive) remained relatively unchanged. The relative increase in peroxisomal activities was accompanied by a respective increase in the number of peroxisomes as well as in thymidine incorporation rate.
AB - Peroxisomal activities have been evaluated in cultured rat hepatocytes in the presence of bezafibrate or long-chain fatty acids added to the culture medium. All activities decreased continuously over a time period of 100 h in culture but selected activities were relatively increased as a function of the added effectors. This relative increase in peroxisomal activities was dose-dependent, discernible within the first 24 h in culture and consisted of activities related specifically to peroxisomal fatty acyl β-oxidation, e.g., cyanide-insensitive palmitoyl-CoA oxidation, H2O2-forming palmitoyl-CoA oxidase and heat-labile enoyl-CoA hydratase. Peroxisomal catalase or mitochondrial fatty acyl β-oxidation (cyanide-sensitive) remained relatively unchanged. The relative increase in peroxisomal activities was accompanied by a respective increase in the number of peroxisomes as well as in thymidine incorporation rate.
KW - (Cultured rat hepatocyte)
KW - Bezafihrate
KW - Long-chain fatty acid
KW - Peroxisomal enzyme
KW - Peroxisome proliferation
KW - β-Oxidation
UR - http://www.scopus.com/inward/record.url?scp=0022358190&partnerID=8YFLogxK
U2 - 10.1016/0005-2760(85)90066-9
DO - 10.1016/0005-2760(85)90066-9
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C2 - 2992600
AN - SCOPUS:0022358190
SN - 0005-2760
VL - 836
SP - 192
EP - 200
JO - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
JF - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
IS - 2
ER -