The effect of centrifugal force on the transcription levels of collagen type I and collagenase in cultured canine gingival fibroblasts

Meir Redlich*, Aaron Palmon, Batya Zaks, Edel Geremi, Sofia Rayzman, Shmuel Shoshan

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

An orthodontically treated tooth is often destabilized in its newly corrected location and relapses towards its original position. Hitherto, the explanation for this phenomenon was that orthodontic force brings about 'stretching' of gingival collagen fiber, which 'pull back' the tooth towards its pretreatment position. A previous ultrastructural study showed that after force application the gingival collagen fibres were torn, laterally spaced and of increased diameter. Therefore, they could not 'pull back' the tooth and be the cause of the relapse. In the present study, in order to find a more plausible explanation at the molecular level, the effect of pressure on the gene transcription of collagen type I and tissue collagenase was examined by semiquantitative, reverse transcriptase-polymerase chain reaction assay. Attached buccal gingiva was excised from anaesthetized dogs and gingival fibroblasts were grown in culture. Following application of pressure (0.167 kg/l g cell mass), the transcription of collagen type I was increased while that of tissue collagenase was decreased. These results corroborate the ultrastructural in vivo findings that orthodontic force is associated with larger amounts of collagen type I in the gingiva.

Original languageAmerican English
Pages (from-to)313-316
Number of pages4
JournalArchives of Oral Biology
Volume43
Issue number4
DOIs
StatePublished - 1 Apr 1998
Externally publishedYes

Keywords

  • Collagen
  • Collagenase
  • Force
  • Gene expression

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