The effect of cis-platin analogues derived from aminoalkylaminoanthraquinones on DNA cleavage: an electron microscopy study

Svetlana Kitov*, Raphael Ben-Shoshan, Israel Ringel, Dan Gibson, Jehoshua Katzhendler

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

The effects of cis-diamminedichloroplatinum(II) (cis-DDP) and a new analog (cis-DANAP) derived from aminoalkylaminoanthraquinones (RNH(CH2)3NH2)2PtCl2, R = anthraquinone on the oligonucleotide poly(dG)·poly(dC) were studied by electron microscopy. It was observed that with cis-DANAP, the extent of small fragments increased with the increase of the drug/nucleotide molar ratio (Rb). This effect was not observed with cis-DDP and with the parent aminoalkylaminoanthraquinone free base. The effect was not time dependent and could be observed only at Rb > 0.4. Explanation for this phenomenon was given on the basis of monoadduct formation between the platinum and purine/pyrimidine bases which leads to strand degradation. A salt effect on the length of poly(dG)·poly(dC) is also present.

Original languageEnglish
Pages (from-to)381-383
Number of pages3
JournalEuropean Journal of Medicinal Chemistry
Volume23
Issue number4
DOIs
StatePublished - 1988

Keywords

  • DNA
  • electron microscopy
  • platinum complexes

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