The Effect of Growth Conditions and Genetic Background on Laccase Production by the Fungus Pleurotus ostreatus

L. Homolka; J. Paltiel; I. Voláková; F. Nerud; Y. Hadar

doi:10.1007/BF02826568

The Effect of Growth Conditions and Genetic Background on Laccase Production by the Fungus Pleurotus ostreatus

L. Homolka^*, J. Paltiel, I. Voláková, F. Nerud, Y. Hadar

^*Corresponding author for this work

Department of Plant Pathology and Microbiology

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

The measurement of growth rate of three isolates - P14, P15 and P19 (obtained after regeneration of protoplasts of the control strain) with significantly higher (P14, P19) or lower (P15) activity of laccase than the control strain (P. ostreatus florida F6) revealed similar patterns for P14 and P19; P15 had a higher growth rate and the highest rate was observed in the control strain. The rate of mineralization of ¹⁴C-lignin on SSF (perlite) by strains F6 and P15 was almost the same and it was higher than that of P14 and P19 strains The enzyme pattern of the individual isolates, studied by gel electrophoresis and activity staining of laccase, did not show any difference in isozyme patterns of the isolates, but there were differences in the level of gene expression.

Original language	English
Pages (from-to)	527-529
Number of pages	3
Journal	Folia Microbiologica
Volume	42
Issue number	5
DOIs	https://doi.org/10.1007/BF02826568
State	Published - 1997

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abstract = "The measurement of growth rate of three isolates - P14, P15 and P19 (obtained after regeneration of protoplasts of the control strain) with significantly higher (P14, P19) or lower (P15) activity of laccase than the control strain (P. ostreatus florida F6) revealed similar patterns for P14 and P19; P15 had a higher growth rate and the highest rate was observed in the control strain. The rate of mineralization of 14C-lignin on SSF (perlite) by strains F6 and P15 was almost the same and it was higher than that of P14 and P19 strains The enzyme pattern of the individual isolates, studied by gel electrophoresis and activity staining of laccase, did not show any difference in isozyme patterns of the isolates, but there were differences in the level of gene expression.",

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AU - Homolka, L.

AU - Paltiel, J.

AU - Voláková, I.

AU - Nerud, F.

AU - Hadar, Y.

PY - 1997

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AB - The measurement of growth rate of three isolates - P14, P15 and P19 (obtained after regeneration of protoplasts of the control strain) with significantly higher (P14, P19) or lower (P15) activity of laccase than the control strain (P. ostreatus florida F6) revealed similar patterns for P14 and P19; P15 had a higher growth rate and the highest rate was observed in the control strain. The rate of mineralization of 14C-lignin on SSF (perlite) by strains F6 and P15 was almost the same and it was higher than that of P14 and P19 strains The enzyme pattern of the individual isolates, studied by gel electrophoresis and activity staining of laccase, did not show any difference in isozyme patterns of the isolates, but there were differences in the level of gene expression.

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The Effect of Growth Conditions and Genetic Background on Laccase Production by the Fungus Pleurotus ostreatus

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