The effect of local attachment of cationized antioxidant enzymes on experimental colitis in the rat

S. Blau; R. Kohen; P. Bass; A. Rubinstein

doi:10.1023/A:1026401611961

The effect of local attachment of cationized antioxidant enzymes on experimental colitis in the rat

S. Blau, R. Kohen^*, P. Bass, A. Rubinstein

^*Corresponding author for this work

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Purpose. To investigate the possibility of local treatment of colitis with the adhesive antioxidant enzymes catalase and superoxide dismutase (SOD). Methods. The net electric charge of the enzymes' surfaces was modified from negative to positive, to cause their adherence to the colon epithelium. The effects of this local administration were assessed in inflamed rat colon. Inflammation severity (colitis) was assessed by measuring colonic tissue myeloperoxidase (MPO) activity, amounts of tumour necrosis factor alpha (TNFα) and concentrations of reduced glutathione (GSH). The measurements were carried out in two types of protocols: preventive (pre-colitis induction) and treatment (post-colitis induction). In addition, the efficacy of treatment with the cationized enzymes was compared to 5-aminosalicylic acid (5-ASA) and betamethasone with similar administration routes. Results. The two cationized antioxidant enzymes were found to be efficient in both prevention and treatment of experimental colitis. The two cationized enzymes caused a significant reduction in MPO activity. A reduction in TNFα concentration was noted only after the treatment protocol. No correlation was found between inflammation severity and tissue levels of GSH. In most cases the cationized enzymes were more effective than 5-ASA and betamethasone. Conclusion. Cationized catalase and cationized SOD have the potential to be efficient therapeutic tools in the local treatment of colitis.

Original language	English
Pages (from-to)	1077-1084
Number of pages	8
Journal	Pharmaceutical Research
Volume	17
Issue number	9
DOIs	https://doi.org/10.1023/A:1026401611961
State	Published - 2000

Bibliographical note

Funding Information:
The study was supported by a research grant from the American-Israeli Binational Foundation number 93-56. The results reported here are included in the dissertation thesis of S.B. as partial fulfilment of her Ph.D. degree requirements at The Hebrew University of Jerusalem. The helpful discussions, comments, and good will of Professor Miles Epstein, Department of Anatomy, University of Wisconsin Medical School and the skillful technical assistance of Ms. Naama Levin are greatly acknowledged.

Keywords

5-aminosalicylic acid (5-ASA)
Adhesive antioxidant enzymes
Betamethasone
Catalase
Cationization
Experimental colitis
Local attachment
Superoxide dismutase (SOD)

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10.1023/A:1026401611961

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title = "The effect of local attachment of cationized antioxidant enzymes on experimental colitis in the rat",

abstract = "Purpose. To investigate the possibility of local treatment of colitis with the adhesive antioxidant enzymes catalase and superoxide dismutase (SOD). Methods. The net electric charge of the enzymes' surfaces was modified from negative to positive, to cause their adherence to the colon epithelium. The effects of this local administration were assessed in inflamed rat colon. Inflammation severity (colitis) was assessed by measuring colonic tissue myeloperoxidase (MPO) activity, amounts of tumour necrosis factor alpha (TNFα) and concentrations of reduced glutathione (GSH). The measurements were carried out in two types of protocols: preventive (pre-colitis induction) and treatment (post-colitis induction). In addition, the efficacy of treatment with the cationized enzymes was compared to 5-aminosalicylic acid (5-ASA) and betamethasone with similar administration routes. Results. The two cationized antioxidant enzymes were found to be efficient in both prevention and treatment of experimental colitis. The two cationized enzymes caused a significant reduction in MPO activity. A reduction in TNFα concentration was noted only after the treatment protocol. No correlation was found between inflammation severity and tissue levels of GSH. In most cases the cationized enzymes were more effective than 5-ASA and betamethasone. Conclusion. Cationized catalase and cationized SOD have the potential to be efficient therapeutic tools in the local treatment of colitis.",

keywords = "5-aminosalicylic acid (5-ASA), Adhesive antioxidant enzymes, Betamethasone, Catalase, Cationization, Experimental colitis, Local attachment, Superoxide dismutase (SOD)",

author = "S. Blau and R. Kohen and P. Bass and A. Rubinstein",

note = "Funding Information: The study was supported by a research grant from the American-Israeli Binational Foundation number 93-56. The results reported here are included in the dissertation thesis of S.B. as partial fulfilment of her Ph.D. degree requirements at The Hebrew University of Jerusalem. The helpful discussions, comments, and good will of Professor Miles Epstein, Department of Anatomy, University of Wisconsin Medical School and the skillful technical assistance of Ms. Naama Levin are greatly acknowledged.",

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AU - Kohen, R.

AU - Bass, P.

AU - Rubinstein, A.

N1 - Funding Information: The study was supported by a research grant from the American-Israeli Binational Foundation number 93-56. The results reported here are included in the dissertation thesis of S.B. as partial fulfilment of her Ph.D. degree requirements at The Hebrew University of Jerusalem. The helpful discussions, comments, and good will of Professor Miles Epstein, Department of Anatomy, University of Wisconsin Medical School and the skillful technical assistance of Ms. Naama Levin are greatly acknowledged.

PY - 2000

Y1 - 2000

N2 - Purpose. To investigate the possibility of local treatment of colitis with the adhesive antioxidant enzymes catalase and superoxide dismutase (SOD). Methods. The net electric charge of the enzymes' surfaces was modified from negative to positive, to cause their adherence to the colon epithelium. The effects of this local administration were assessed in inflamed rat colon. Inflammation severity (colitis) was assessed by measuring colonic tissue myeloperoxidase (MPO) activity, amounts of tumour necrosis factor alpha (TNFα) and concentrations of reduced glutathione (GSH). The measurements were carried out in two types of protocols: preventive (pre-colitis induction) and treatment (post-colitis induction). In addition, the efficacy of treatment with the cationized enzymes was compared to 5-aminosalicylic acid (5-ASA) and betamethasone with similar administration routes. Results. The two cationized antioxidant enzymes were found to be efficient in both prevention and treatment of experimental colitis. The two cationized enzymes caused a significant reduction in MPO activity. A reduction in TNFα concentration was noted only after the treatment protocol. No correlation was found between inflammation severity and tissue levels of GSH. In most cases the cationized enzymes were more effective than 5-ASA and betamethasone. Conclusion. Cationized catalase and cationized SOD have the potential to be efficient therapeutic tools in the local treatment of colitis.

AB - Purpose. To investigate the possibility of local treatment of colitis with the adhesive antioxidant enzymes catalase and superoxide dismutase (SOD). Methods. The net electric charge of the enzymes' surfaces was modified from negative to positive, to cause their adherence to the colon epithelium. The effects of this local administration were assessed in inflamed rat colon. Inflammation severity (colitis) was assessed by measuring colonic tissue myeloperoxidase (MPO) activity, amounts of tumour necrosis factor alpha (TNFα) and concentrations of reduced glutathione (GSH). The measurements were carried out in two types of protocols: preventive (pre-colitis induction) and treatment (post-colitis induction). In addition, the efficacy of treatment with the cationized enzymes was compared to 5-aminosalicylic acid (5-ASA) and betamethasone with similar administration routes. Results. The two cationized antioxidant enzymes were found to be efficient in both prevention and treatment of experimental colitis. The two cationized enzymes caused a significant reduction in MPO activity. A reduction in TNFα concentration was noted only after the treatment protocol. No correlation was found between inflammation severity and tissue levels of GSH. In most cases the cationized enzymes were more effective than 5-ASA and betamethasone. Conclusion. Cationized catalase and cationized SOD have the potential to be efficient therapeutic tools in the local treatment of colitis.

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The effect of local attachment of cationized antioxidant enzymes on experimental colitis in the rat

Abstract

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Keywords

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