TY - JOUR
T1 - The effect of macrophage hydrolytic enzyme levels on the uptake and degradation of antigen and immune complexes
AU - Bar Eli, M.
AU - Gallily, R.
PY - 1975
Y1 - 1975
N2 - Macrophages isolated from the peritoneal cavity of mice stimulated with thioglycollate, methanol extraction residue of tubercle bacilli (MER), Bayol F or sodium caseinate demonstrated increased levels of acid phosphatase, β glucuronidase, cathepsin D and lysozyme as compared with unstimulated cells. Elevated enzyme levels were characteristic for the stimulant used. 125I labeled Shigella and 125I labeled poly L (Tyr Glu) poly L pro poly L Lys [(T,G) PRO L] were endocytosed much more slowly by stimulated macrophages. Degradation of Shigella in these stimulated cells was much faster than in unstimulated cells, while degradation of (T,G) PRO L was slower in stimulated cells than in unstimulated ones. Thus, no direct correlation between higher lysosomal enzyme levels and macrophage degradative capacity could be demonstrated. Immune complexes were engulfed by unstimulated and stimulated macrophages much faster than were uncomplexed antigens (Ags), while their degradation was generally slower than that of the Ag alone.
AB - Macrophages isolated from the peritoneal cavity of mice stimulated with thioglycollate, methanol extraction residue of tubercle bacilli (MER), Bayol F or sodium caseinate demonstrated increased levels of acid phosphatase, β glucuronidase, cathepsin D and lysozyme as compared with unstimulated cells. Elevated enzyme levels were characteristic for the stimulant used. 125I labeled Shigella and 125I labeled poly L (Tyr Glu) poly L pro poly L Lys [(T,G) PRO L] were endocytosed much more slowly by stimulated macrophages. Degradation of Shigella in these stimulated cells was much faster than in unstimulated cells, while degradation of (T,G) PRO L was slower in stimulated cells than in unstimulated ones. Thus, no direct correlation between higher lysosomal enzyme levels and macrophage degradative capacity could be demonstrated. Immune complexes were engulfed by unstimulated and stimulated macrophages much faster than were uncomplexed antigens (Ags), while their degradation was generally slower than that of the Ag alone.
UR - http://www.scopus.com/inward/record.url?scp=0016714629&partnerID=8YFLogxK
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C2 - 1214262
AN - SCOPUS:0016714629
SN - 0033-6890
VL - 18
SP - 317
EP - 328
JO - RES Journal of the Reticuloendothelial Society
JF - RES Journal of the Reticuloendothelial Society
IS - 6
ER -