The effect of medium constituents on growth and diosgenin production by Dioscorea deltoidea cells grown in batch cultures

B Tal, J Gressel, I Goldberg

Research output: Contribution to journalArticlepeer-review

Abstract

The effect of nitrogen and carbon sources on growth and diosgenin production was studied with Dioscorea deltoidea cells which were grown under defined conditions in a chemostat, and transferred to batch cultures. Both ammonium and nitrate nitrogen were required for growth and steroid production; diosgenin production was maximal at higher amounts of nitrogen. The nature and concentration of the carbon source (sugar) had marked effects on diosgenin production. A maximal amount of diosgenin (3.8% of dry wt.) was produced on sucrose (15 g/liter) after 21 days of growth in a batch culture.

Original languageEnglish
Pages (from-to)111-115
Number of pages5
JournalPlanta Medica
Volume44
Issue number2
DOIs
StatePublished - Feb 1982

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