TY - JOUR
T1 - The effect of urea, sodium and calcium on microsomal ATPase activity in different parts of the kidney
AU - Gutman, Yehuda
AU - Katzper-Shamir, Yaffa
PY - 1971/3/9
Y1 - 1971/3/9
N2 - Mg2+-ATPase and (Na+ + K+)-dependent ATPase activities were assayed in the microsomal fractions of the cortex, medulla and papilla of the guinea-pig kidney. Mg2+-ATPase in any part of the kidney was unchanged in the presence of 300 mM urea. (Na+ + K+)-dependent ATPase activity was unchanged in the cortex, but was significantly inhibited in the medulla and papilla by 300 mM urea. Elevated concentrations of Na+ (200 mM) depressed ATPase activity in the microsomal fraction derived from the papilla and medulla, but did not significantly affect the activity in the kidney cortex. Ca2+ (5 mM) caused a significant inhibition of (Na+ + K+)-dependent ATPase in all three parts of the kidney and depressed Mg2+-ATPase activity only in the papilla.
AB - Mg2+-ATPase and (Na+ + K+)-dependent ATPase activities were assayed in the microsomal fractions of the cortex, medulla and papilla of the guinea-pig kidney. Mg2+-ATPase in any part of the kidney was unchanged in the presence of 300 mM urea. (Na+ + K+)-dependent ATPase activity was unchanged in the cortex, but was significantly inhibited in the medulla and papilla by 300 mM urea. Elevated concentrations of Na+ (200 mM) depressed ATPase activity in the microsomal fraction derived from the papilla and medulla, but did not significantly affect the activity in the kidney cortex. Ca2+ (5 mM) caused a significant inhibition of (Na+ + K+)-dependent ATPase in all three parts of the kidney and depressed Mg2+-ATPase activity only in the papilla.
UR - http://www.scopus.com/inward/record.url?scp=0015216104&partnerID=8YFLogxK
U2 - 10.1016/0005-2736(71)90365-8
DO - 10.1016/0005-2736(71)90365-8
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C2 - 4252906
AN - SCOPUS:0015216104
SN - 0005-2736
VL - 233
SP - 133
EP - 136
JO - Biochimica et Biophysica Acta - Biomembranes
JF - Biochimica et Biophysica Acta - Biomembranes
IS - 1
ER -
