The effect of urea, sodium and calcium on microsomal ATPase activity in different parts of the kidney

Yehuda Gutman; Yaffa Katzper-Shamir

doi:10.1016/0005-2736(71)90365-8

The effect of urea, sodium and calcium on microsomal ATPase activity in different parts of the kidney

Yehuda Gutman^*
, Yaffa Katzper-Shamir

^*Corresponding author for this work

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

Mg²⁺-ATPase and (Na⁺ + K⁺)-dependent ATPase activities were assayed in the microsomal fractions of the cortex, medulla and papilla of the guinea-pig kidney. Mg²⁺-ATPase in any part of the kidney was unchanged in the presence of 300 mM urea. (Na⁺ + K⁺)-dependent ATPase activity was unchanged in the cortex, but was significantly inhibited in the medulla and papilla by 300 mM urea. Elevated concentrations of Na⁺ (200 mM) depressed ATPase activity in the microsomal fraction derived from the papilla and medulla, but did not significantly affect the activity in the kidney cortex. Ca²⁺ (5 mM) caused a significant inhibition of (Na⁺ + K⁺)-dependent ATPase in all three parts of the kidney and depressed Mg²⁺-ATPase activity only in the papilla.

Original language	English
Pages (from-to)	133-136
Number of pages	4
Journal	Biochimica et Biophysica Acta - Biomembranes
Volume	233
Issue number	1
DOIs	https://doi.org/10.1016/0005-2736(71)90365-8
State	Published - 9 Mar 1971

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abstract = "Mg2+-ATPase and (Na+ + K+)-dependent ATPase activities were assayed in the microsomal fractions of the cortex, medulla and papilla of the guinea-pig kidney. Mg2+-ATPase in any part of the kidney was unchanged in the presence of 300 mM urea. (Na+ + K+)-dependent ATPase activity was unchanged in the cortex, but was significantly inhibited in the medulla and papilla by 300 mM urea. Elevated concentrations of Na+ (200 mM) depressed ATPase activity in the microsomal fraction derived from the papilla and medulla, but did not significantly affect the activity in the kidney cortex. Ca2+ (5 mM) caused a significant inhibition of (Na+ + K+)-dependent ATPase in all three parts of the kidney and depressed Mg2+-ATPase activity only in the papilla.",

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N2 - Mg2+-ATPase and (Na+ + K+)-dependent ATPase activities were assayed in the microsomal fractions of the cortex, medulla and papilla of the guinea-pig kidney. Mg2+-ATPase in any part of the kidney was unchanged in the presence of 300 mM urea. (Na+ + K+)-dependent ATPase activity was unchanged in the cortex, but was significantly inhibited in the medulla and papilla by 300 mM urea. Elevated concentrations of Na+ (200 mM) depressed ATPase activity in the microsomal fraction derived from the papilla and medulla, but did not significantly affect the activity in the kidney cortex. Ca2+ (5 mM) caused a significant inhibition of (Na+ + K+)-dependent ATPase in all three parts of the kidney and depressed Mg2+-ATPase activity only in the papilla.

AB - Mg2+-ATPase and (Na+ + K+)-dependent ATPase activities were assayed in the microsomal fractions of the cortex, medulla and papilla of the guinea-pig kidney. Mg2+-ATPase in any part of the kidney was unchanged in the presence of 300 mM urea. (Na+ + K+)-dependent ATPase activity was unchanged in the cortex, but was significantly inhibited in the medulla and papilla by 300 mM urea. Elevated concentrations of Na+ (200 mM) depressed ATPase activity in the microsomal fraction derived from the papilla and medulla, but did not significantly affect the activity in the kidney cortex. Ca2+ (5 mM) caused a significant inhibition of (Na+ + K+)-dependent ATPase in all three parts of the kidney and depressed Mg2+-ATPase activity only in the papilla.

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The effect of urea, sodium and calcium on microsomal ATPase activity in different parts of the kidney

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