The Escherichia coli OxyS regulatory RNA represses fhlA translation by blocking ribosome binding

Shoshy Altuvia, Aixia Zhang, Liron Argaman, Anita Tiwari, Gisela Storz*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

191 Scopus citations

Abstract

OxyS is a small untranslated RNA which is induced in response to oxidative stress in Escherichia coli. This novel RNA acts as a global regulator to activate or repress the expression of as many as 40 genes, including the fhlA encoded transcriptional activator and the rpoS-encoded σ(s) subunit of RNA polymerase. Deletion analysis of OxyS showed that different domains of the small RNA are required for the regulation of fhlA and rpoS. We examined the mechanism of OxyS repression of fhlA and found that the OxyS RNA inhibits fhlA translation by pairing with a short sequence overlapping the Shine-Dalgarno sequence, thereby blocking ribosome binding/translation.

Original languageAmerican English
Pages (from-to)6069-6075
Number of pages7
JournalEMBO Journal
Volume17
Issue number20
DOIs
StatePublished - 15 Oct 1998

Keywords

  • Antisense regulation
  • Oxidative stress
  • Oxys RNA
  • Ribosome binding
  • Translation

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