The fate of IgM and of anti IgM on antibody-coated Daudi cells

Eitan Yefenof; Isaac P. Witz; E.  Klein

The fate of IgM and of anti IgM on antibody-coated Daudi cells

Eitan Yefenof, Isaac P. Witz, E. Klein

Institute for Medical Research Israel-Canada (IMRIC)

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

This chapter discusses an experiment to show the fate of immunoglobulin M (IgM) and anti-immunoglobulin M (anti- IgM) antibodies on antibody-coated Daudi cells. In the experiment, antihuman IgM (aIgM) antibodies were purified by the affinity chromatography of a sheep aIgM antiserum on IgM-conjugated sepharose columns. Daudi cells were coated with 125I labeled aIgM (125I-algM) and incubated under culture conditions at a temperature of 37°C. It resulted that more than 80% of the cell-bound radioactivity was shed from the cells into the culture medium within 10 hours. From the experiment, it was found that 125I-aIgM shed from Daudi cells could rebind to fresh Daudi cells but less efficiently than native 125I-aIgM. The shedding of aIgM from Daudi cells resulted in the disappearance of the IgM molecules from the cell membrane and in the formation of IgM–aIgM complexes. Shed aIgM antibodies were partially degraded during the binding to the cell membrane and shedding.

Original language	English
Title of host publication	Leukocyte membrane determinants regulating immune reactivity
Subtitle of host publication	proceedings of the Tenth Leukocyte Culture Conference, held in Amsterdam, the Netherlands, September 10-13, 1975
Editors	Vincent P. Eijsvoogel, Dirk Roos, Wim P. Zeijlemaker
Place of Publication	New York
Publisher	Academic Press
Pages	109-110
ISBN (Print)	0122337506, 9780122337505
State	Published - 1976

Publication series

Name	Proceedings of the Leukocyte Culture Conference
Volume	10

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Yefenof, E., Witz, I. P., & Klein, E. (1976). The fate of IgM and of anti IgM on antibody-coated Daudi cells. In V. P. Eijsvoogel, D. Roos, & W. P. Zeijlemaker (Eds.), Leukocyte membrane determinants regulating immune reactivity : proceedings of the Tenth Leukocyte Culture Conference, held in Amsterdam, the Netherlands, September 10-13, 1975 (pp. 109-110). (Proceedings of the Leukocyte Culture Conference; Vol. 10). Academic Press.

Yefenof, Eitan ; Witz, Isaac P. ; Klein, E. . / The fate of IgM and of anti IgM on antibody-coated Daudi cells. Leukocyte membrane determinants regulating immune reactivity : proceedings of the Tenth Leukocyte Culture Conference, held in Amsterdam, the Netherlands, September 10-13, 1975. editor / Vincent P. Eijsvoogel ; Dirk Roos ; Wim P. Zeijlemaker. New York : Academic Press, 1976. pp. 109-110 (Proceedings of the Leukocyte Culture Conference).

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abstract = "This chapter discusses an experiment to show the fate of immunoglobulin M (IgM) and anti-immunoglobulin M (anti- IgM) antibodies on antibody-coated Daudi cells. In the experiment, antihuman IgM (aIgM) antibodies were purified by the affinity chromatography of a sheep aIgM antiserum on IgM-conjugated sepharose columns. Daudi cells were coated with 125I labeled aIgM (125I-algM) and incubated under culture conditions at a temperature of 37°C. It resulted that more than 80\% of the cell-bound radioactivity was shed from the cells into the culture medium within 10 hours. From the experiment, it was found that 125I-aIgM shed from Daudi cells could rebind to fresh Daudi cells but less efficiently than native 125I-aIgM. The shedding of aIgM from Daudi cells resulted in the disappearance of the IgM molecules from the cell membrane and in the formation of IgM–aIgM complexes. Shed aIgM antibodies were partially degraded during the binding to the cell membrane and shedding.",

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Yefenof, E, Witz, IP & Klein, E 1976, The fate of IgM and of anti IgM on antibody-coated Daudi cells. in VP Eijsvoogel, D Roos & WP Zeijlemaker (eds), Leukocyte membrane determinants regulating immune reactivity : proceedings of the Tenth Leukocyte Culture Conference, held in Amsterdam, the Netherlands, September 10-13, 1975. Proceedings of the Leukocyte Culture Conference, vol. 10, Academic Press, New York, pp. 109-110.

The fate of IgM and of anti IgM on antibody-coated Daudi cells. / Yefenof, Eitan; Witz, Isaac P.; Klein, E. .
Leukocyte membrane determinants regulating immune reactivity : proceedings of the Tenth Leukocyte Culture Conference, held in Amsterdam, the Netherlands, September 10-13, 1975. ed. / Vincent P. Eijsvoogel; Dirk Roos; Wim P. Zeijlemaker. New York: Academic Press, 1976. p. 109-110 (Proceedings of the Leukocyte Culture Conference; Vol. 10).

Research output: Chapter in Book/Report/Conference proceeding › Chapter

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T1 - The fate of IgM and of anti IgM on antibody-coated Daudi cells

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AU - Witz, Isaac P.

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N2 - This chapter discusses an experiment to show the fate of immunoglobulin M (IgM) and anti-immunoglobulin M (anti- IgM) antibodies on antibody-coated Daudi cells. In the experiment, antihuman IgM (aIgM) antibodies were purified by the affinity chromatography of a sheep aIgM antiserum on IgM-conjugated sepharose columns. Daudi cells were coated with 125I labeled aIgM (125I-algM) and incubated under culture conditions at a temperature of 37°C. It resulted that more than 80% of the cell-bound radioactivity was shed from the cells into the culture medium within 10 hours. From the experiment, it was found that 125I-aIgM shed from Daudi cells could rebind to fresh Daudi cells but less efficiently than native 125I-aIgM. The shedding of aIgM from Daudi cells resulted in the disappearance of the IgM molecules from the cell membrane and in the formation of IgM–aIgM complexes. Shed aIgM antibodies were partially degraded during the binding to the cell membrane and shedding.

AB - This chapter discusses an experiment to show the fate of immunoglobulin M (IgM) and anti-immunoglobulin M (anti- IgM) antibodies on antibody-coated Daudi cells. In the experiment, antihuman IgM (aIgM) antibodies were purified by the affinity chromatography of a sheep aIgM antiserum on IgM-conjugated sepharose columns. Daudi cells were coated with 125I labeled aIgM (125I-algM) and incubated under culture conditions at a temperature of 37°C. It resulted that more than 80% of the cell-bound radioactivity was shed from the cells into the culture medium within 10 hours. From the experiment, it was found that 125I-aIgM shed from Daudi cells could rebind to fresh Daudi cells but less efficiently than native 125I-aIgM. The shedding of aIgM from Daudi cells resulted in the disappearance of the IgM molecules from the cell membrane and in the formation of IgM–aIgM complexes. Shed aIgM antibodies were partially degraded during the binding to the cell membrane and shedding.

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Yefenof E, Witz IP, Klein E. The fate of IgM and of anti IgM on antibody-coated Daudi cells. In Eijsvoogel VP, Roos D, Zeijlemaker WP, editors, Leukocyte membrane determinants regulating immune reactivity : proceedings of the Tenth Leukocyte Culture Conference, held in Amsterdam, the Netherlands, September 10-13, 1975. New York: Academic Press. 1976. p. 109-110. (Proceedings of the Leukocyte Culture Conference).

The fate of IgM and of anti IgM on antibody-coated Daudi cells

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