TY - JOUR
T1 - The formation of stable E rosettes by human T lymphocytes activated in mixed lymphocyte reactions
AU - Galili, U.
AU - Schlesinger, M.
PY - 1976
Y1 - 1976
N2 - The aim of the present study was to determine whether activation of human T lymphocytes affects their interaction with sheep red blood cells (SRBC). Less than 3% of the E rosettes formed by freshly isolated peripheral blood lymphocytes (PBL) and SRBC are stable and do not disintegrate after incubation at 37°C. In contrast, about 30% of PBL kept in culture for 5 days in the presence of mitomycin C treated allogeneic lymphocytes were found to form stable E rosettes. Whereas no rosettes were formed by freshly isolated PBL incubated with human red blood cells at 24°C, 15% of the cells recovered from mixed lymphocyte reactions (MLR) formed such rosettes. When responder PBL were maintained in culture in the absence of allogeneic stimuli the proportion of cells forming stable E rosettes depended on the serum present in the medium. Less than 5% of the responder cells kept in medium containing human serum or in serum free medium formed stable E rosettes, whereas 18% of the cells maintained in medium containing fetal calf serum formed stable E rosettes. The proportion of cells forming stable E rosettes increased before any increase in DNA synthesis was detectable in MLR. Indeed, a high proportion of cells forming stable E rosettes appeared in MLR taking place in serum free medium, without any accompanying increase of DNA synthesis. Depletion of cells forming EAC' rosettes from responder PBL increased the proportion of cells forming stable E rosettes in MLR. Exposure of the cells recovered in MLR to specific anti T sera inhibited the formation of both stable and regular E rosettes. Exposure of the cells recovered in MLR to anti Ig serum had no effect on the formation of regular rosettes. Anti Ig serum strongly inhibited the formation of stable E rosettes by cells grown in medium containing human serum, but had no effect on the formation of stable E rosettes by cells grown in either serum free medium or in serum containing fetal calf serum. It is concluded that activated human T lymphocytes are characterized by their capacity to form stable E rosettes, resistant to incubation at 37°C, and by their capacity to acquire an immunoglobulin coat, possibly by binding immunoglobulin molecules present in their environment.
AB - The aim of the present study was to determine whether activation of human T lymphocytes affects their interaction with sheep red blood cells (SRBC). Less than 3% of the E rosettes formed by freshly isolated peripheral blood lymphocytes (PBL) and SRBC are stable and do not disintegrate after incubation at 37°C. In contrast, about 30% of PBL kept in culture for 5 days in the presence of mitomycin C treated allogeneic lymphocytes were found to form stable E rosettes. Whereas no rosettes were formed by freshly isolated PBL incubated with human red blood cells at 24°C, 15% of the cells recovered from mixed lymphocyte reactions (MLR) formed such rosettes. When responder PBL were maintained in culture in the absence of allogeneic stimuli the proportion of cells forming stable E rosettes depended on the serum present in the medium. Less than 5% of the responder cells kept in medium containing human serum or in serum free medium formed stable E rosettes, whereas 18% of the cells maintained in medium containing fetal calf serum formed stable E rosettes. The proportion of cells forming stable E rosettes increased before any increase in DNA synthesis was detectable in MLR. Indeed, a high proportion of cells forming stable E rosettes appeared in MLR taking place in serum free medium, without any accompanying increase of DNA synthesis. Depletion of cells forming EAC' rosettes from responder PBL increased the proportion of cells forming stable E rosettes in MLR. Exposure of the cells recovered in MLR to specific anti T sera inhibited the formation of both stable and regular E rosettes. Exposure of the cells recovered in MLR to anti Ig serum had no effect on the formation of regular rosettes. Anti Ig serum strongly inhibited the formation of stable E rosettes by cells grown in medium containing human serum, but had no effect on the formation of stable E rosettes by cells grown in either serum free medium or in serum containing fetal calf serum. It is concluded that activated human T lymphocytes are characterized by their capacity to form stable E rosettes, resistant to incubation at 37°C, and by their capacity to acquire an immunoglobulin coat, possibly by binding immunoglobulin molecules present in their environment.
UR - http://www.scopus.com/inward/record.url?scp=0017200347&partnerID=8YFLogxK
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C2 - 134120
AN - SCOPUS:0017200347
SN - 0022-1767
VL - 117
SP - 730
EP - 735
JO - Journal of Immunology
JF - Journal of Immunology
IS - 3
ER -