TY - JOUR
T1 - The function of H and L chains in iron sequestration in ferritin
AU - Bauminger, E. R.
AU - Harrison, P. M.
AU - Hechel, D.
AU - Nowik, I.
AU - Treffry, A.
PY - 1993/4/4
Y1 - 1993/4/4
N2 - In order to understand the iron sequestering mechanism within the protein shell in ferritins, Mössbauer studies were performed on horse spleen ferritin (about 15% H and 85% L chains) and variants of human H chain ferritins in which putative oxidation and nucleation site ligands have been changed by site directed mutagenesis and which were loaded with small amounts of iron and frozen at short times after iron loading. It was found that the catalysis by apoferritin of Fe(II) oxidation is associated with residues within the H chains, that both Fe(III) monomers and dimers are located on H chains and that these dimers form at ferroxidase centers. Nevertheless iron core formation does occur also in ferritins that lack the ferroxidase center of the H chains, though initial Fe(II) oxidation is slower.
AB - In order to understand the iron sequestering mechanism within the protein shell in ferritins, Mössbauer studies were performed on horse spleen ferritin (about 15% H and 85% L chains) and variants of human H chain ferritins in which putative oxidation and nucleation site ligands have been changed by site directed mutagenesis and which were loaded with small amounts of iron and frozen at short times after iron loading. It was found that the catalysis by apoferritin of Fe(II) oxidation is associated with residues within the H chains, that both Fe(III) monomers and dimers are located on H chains and that these dimers form at ferroxidase centers. Nevertheless iron core formation does occur also in ferritins that lack the ferroxidase center of the H chains, though initial Fe(II) oxidation is slower.
UR - http://www.scopus.com/inward/record.url?scp=0040043309&partnerID=8YFLogxK
U2 - 10.1016/0168-583X(93)95251-Y
DO - 10.1016/0168-583X(93)95251-Y
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AN - SCOPUS:0040043309
SN - 0168-583X
VL - 76
SP - 403
EP - 404
JO - Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms
JF - Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms
IS - 1-4
ER -