The genetic basis for the adaptation of E. coli to sugar synthesis from CO2

Elad Herz, Niv Antonovsky, Yinon Bar-On, Dan Davidi, Shmuel Gleizer, Noam Prywes, Lianet Noda-Garcia, Keren Lyn Frisch, Yehudit Zohar, David G. Wernick, Alon Savidor, Uri Barenholz, Ron Milo*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

Understanding the evolution of a new metabolic capability in full mechanistic detail is challenging, as causative mutations may be masked by non-essential "hitchhiking" mutations accumulated during the evolutionary trajectory. We have previously used adaptive laboratory evolution of a rationally engineered ancestor to generate an Escherichia coli strain able to utilize CO2 fixation for sugar synthesis. Here, we reveal the genetic basis underlying this metabolic transition. Five mutations are sufficient to enable robust growth when a non-native Calvin-Benson-Bassham cycle provides all the sugar-derived metabolic building blocks. These mutations are found either in enzymes that affect the efflux of intermediates from the autocatalytic CO2 fixation cycle toward biomass (prs, serA, and pgi), or in key regulators of carbon metabolism (crp and ppsR). Using suppressor analysis, we show that a decrease in catalytic capacity is a common feature of all mutations found in enzymes. These findings highlight the enzymatic constraints that are essential to the metabolic stability of autocatalytic cycles and are relevant to future efforts in constructing non-native carbon fixation pathways.

Original languageAmerican English
Article number1705
JournalNature Communications
Volume8
Issue number1
DOIs
StatePublished - 1 Dec 2017
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2017 The Author(s).

Fingerprint

Dive into the research topics of 'The genetic basis for the adaptation of E. coli to sugar synthesis from CO2'. Together they form a unique fingerprint.

Cite this