The glycogen-amylase complex as a means of obtaining highly purified α-amylases

Abraham Loyter*, Michael Schramm

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

146 Scopus citations

Abstract

α-Amylases of hog pancreas, human saliva and rat parotis are specifically precipitated from crude extracts as a glycogen-enzyme complex insoluble in 40% ethanol. The specific activity (units/mg protein) of the enzymes thus precipitated approaches or is equal to that of the best crystalleine prepations. The procedure is readily carried out on a micro-scale. A method for the removal of glycogen from the complex, with the subsequent preparation of the crystalline pancreatic and parotis enzymes, is described. In the case of pancreatic amylase the enzyme-glycogen complex contains one mole of enzyme per 550 anhydroglucose units.

Original languageEnglish
Pages (from-to)200-206
Number of pages7
JournalBiochimica et Biophysica Acta - General Subjects
Volume65
Issue number2
DOIs
StatePublished - 4 Dec 1962

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