TY - JOUR
T1 - The GUL-1 Protein Binds Multiple RNAs Involved in Cell Wall Remodeling and Affects the MAK-1 Pathway in Neurospora crassa
AU - Herold, Inbal
AU - Zolti, Avihai
AU - Garduño-Rosales, Marisela
AU - Wang, Zheng
AU - López-Giráldez, Francesc
AU - Mouriño-Pérez, Rosa R.
AU - Townsend, Jeffrey P.
AU - Ulitsky, Igor
AU - Yarden, Oded
N1 - Publisher Copyright:
Copyright © 2021 Herold, Zolti, Garduño-Rosales, Wang, López-Giráldez, Mouriño-Pérez, Townsend, Ulitsky and Yarden.
PY - 2021
Y1 - 2021
N2 - The Neurospora crassa GUL-1 is part of the COT-1 pathway, which plays key roles in regulating polar hyphal growth and cell wall remodeling. We show that GUL-1 is a bona fide RNA-binding protein (RBP) that can associate with 828 “core” mRNA species. When cell wall integrity (CWI) is challenged, expression of over 25% of genomic RNA species are modulated (2,628 mRNAs, including the GUL-1 mRNA). GUL-1 binds mRNAs of genes related to translation, cell wall remodeling, circadian clock, endoplasmic reticulum (ER), as well as CWI and MAPK pathway components. GUL-1 interacts with over 100 different proteins, including stress-granule and P-body proteins, ER components and components of the MAPK, COT-1, and STRIPAK complexes. Several additional RBPs were also shown to physically interact with GUL-1. Under stress conditions, GUL-1 can localize to the ER and affect the CWI pathway—evident via altered phosphorylation levels of MAK-1, interaction with mak-1 transcript, and involvement in the expression level of the transcription factor adv-1. We conclude that GUL-1 functions in multiple cellular processes, including the regulation of cell wall remodeling, via a mechanism associated with the MAK-1 pathway and stress-response.
AB - The Neurospora crassa GUL-1 is part of the COT-1 pathway, which plays key roles in regulating polar hyphal growth and cell wall remodeling. We show that GUL-1 is a bona fide RNA-binding protein (RBP) that can associate with 828 “core” mRNA species. When cell wall integrity (CWI) is challenged, expression of over 25% of genomic RNA species are modulated (2,628 mRNAs, including the GUL-1 mRNA). GUL-1 binds mRNAs of genes related to translation, cell wall remodeling, circadian clock, endoplasmic reticulum (ER), as well as CWI and MAPK pathway components. GUL-1 interacts with over 100 different proteins, including stress-granule and P-body proteins, ER components and components of the MAPK, COT-1, and STRIPAK complexes. Several additional RBPs were also shown to physically interact with GUL-1. Under stress conditions, GUL-1 can localize to the ER and affect the CWI pathway—evident via altered phosphorylation levels of MAK-1, interaction with mak-1 transcript, and involvement in the expression level of the transcription factor adv-1. We conclude that GUL-1 functions in multiple cellular processes, including the regulation of cell wall remodeling, via a mechanism associated with the MAK-1 pathway and stress-response.
KW - COT-1
KW - GUL-1
KW - MAPK
KW - Nikkomycin
KW - RNA-binding protein
KW - cell wall integrity pathway
KW - cell wall remodeling
UR - http://www.scopus.com/inward/record.url?scp=85135530591&partnerID=8YFLogxK
U2 - 10.3389/ffunb.2021.672696
DO - 10.3389/ffunb.2021.672696
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
AN - SCOPUS:85135530591
SN - 2673-6128
VL - 2
JO - Frontiers in Fungal Biology
JF - Frontiers in Fungal Biology
M1 - 672696
ER -