The hardwired transcriptional response to DNA damage

Elisheva E. Heilbrun; May Merav; Avital Parnas; Sheera Adar

doi:10.1016/j.coisb.2020.05.001

The hardwired transcriptional response to DNA damage

Elisheva E. Heilbrun, May Merav, Avital Parnas, Sheera Adar^*

^*Corresponding author for this work

Department of Microbiology and Molecular Genetics

Research output: Contribution to journal › Review article › peer-review

3 Scopus citations

Abstract

There is a complex network of interactions between bulky DNA damages and transcription. Bulky damages block RNA polymerases but also elicit a regulated transcriptional response. At the same time, active transcription enhances the ability to recognize and repair damages. Eventually, transcription is completely shut down until after damages are removed. Recent projects untangle this web of interaction in mammalian cells by applying time-sensitive and high-resolution measurements of damage, repair, and transcription at genome-wide scales. The emerging model indicates the transcriptional response to damage is primarily hardwired in the damaged genomic DNA, and transcription shutdown can be explained almost completely by (1) aborted transcription by blocked RNA polymerases and (2) ubiquitination and degradation of RNA polymerase II after encountering a damage.

Original language	English
Pages (from-to)	1-7
Number of pages	7
Journal	Current Opinion in Systems Biology
Volume	19
DOIs	https://doi.org/10.1016/j.coisb.2020.05.001
State	Published - Feb 2020

Bibliographical note

Funding Information:
The authors thank Hadar Golan-Berman for helpful comments. This work was funded by the Israel Science Foundation grants ( 1710/17 ) administered by the Israeli Academy for Science and humanities, the Israel Cancer Association grant ( 20191630 ). SA is the recipient of the Jacob and Lena Joels memorial fund senior lectureship. EEH is the recipient of the Malvina and Solomon Pollack Scholarship fund and the Israel Council for Higher Education scholarship. Figures were generated using BioRender.com .

Funding Information:
The authors thank Hadar Golan-Berman for helpful comments. This work was funded by the Israel Science Foundation grants (1710/17) administered by the Israeli Academy for Science and humanities, the Israel Cancer Association grant (20191630). SA is the recipient of the Jacob and Lena Joels memorial fund senior lectureship. EEH is the recipient of the Malvina and Solomon Pollack Scholarship fund and the Israel Council for Higher Education scholarship. Figures were generated using BioRender.com.

Publisher Copyright:
© 2020 Elsevier Ltd

Keywords

DNA damage
DNA repair
Genomics
Nucleotide excision repair
RNA polymerase II
Transcription
UV
Ubiquitination

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10.1016/j.coisb.2020.05.001

Cite this

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title = "The hardwired transcriptional response to DNA damage",

abstract = "There is a complex network of interactions between bulky DNA damages and transcription. Bulky damages block RNA polymerases but also elicit a regulated transcriptional response. At the same time, active transcription enhances the ability to recognize and repair damages. Eventually, transcription is completely shut down until after damages are removed. Recent projects untangle this web of interaction in mammalian cells by applying time-sensitive and high-resolution measurements of damage, repair, and transcription at genome-wide scales. The emerging model indicates the transcriptional response to damage is primarily hardwired in the damaged genomic DNA, and transcription shutdown can be explained almost completely by (1) aborted transcription by blocked RNA polymerases and (2) ubiquitination and degradation of RNA polymerase II after encountering a damage.",

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AU - Parnas, Avital

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The hardwired transcriptional response to DNA damage

Abstract

Bibliographical note

Keywords

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