TY - JOUR
T1 - The histidine residue in the active centre of ribonuclease
T2 - I. A specific reaction with bromoacetic acid
AU - Barnard, E. A.
AU - Stein, W. D.
PY - 1959
Y1 - 1959
N2 - Bromoacetic acid reacts readily with ribonuclease to form an inactive derivative in which, at pH 7, one carboxymethyl group has been introduced per molecule of enzyme. The sole residue in the enzyme so substituted is shown to be one of the four histidines in the molecule. The rate of reaction of this histidine with bromoacetic acid is far greater than that of free histidine, the residue in the enzyme being, therefore, activated. The reaction with bromoacetic acid is slowed down greatly in the presence of cytidylic acid, a competitive inhibitor of the enzyme, suggesting that the histidine residue concerned is situated in the active centre of the enzyme.
AB - Bromoacetic acid reacts readily with ribonuclease to form an inactive derivative in which, at pH 7, one carboxymethyl group has been introduced per molecule of enzyme. The sole residue in the enzyme so substituted is shown to be one of the four histidines in the molecule. The rate of reaction of this histidine with bromoacetic acid is far greater than that of free histidine, the residue in the enzyme being, therefore, activated. The reaction with bromoacetic acid is slowed down greatly in the presence of cytidylic acid, a competitive inhibitor of the enzyme, suggesting that the histidine residue concerned is situated in the active centre of the enzyme.
UR - http://www.scopus.com/inward/record.url?scp=85025374224&partnerID=8YFLogxK
U2 - 10.1016/S0022-2836(59)80016-4
DO - 10.1016/S0022-2836(59)80016-4
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AN - SCOPUS:85025374224
SN - 0022-2836
VL - 1
SP - 339
EP - 349
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
IS - 4-5
ER -