The impact of alloplast and allograft on bone homeostasis: Orthodontic tooth movement into regenerated bone

Yehuda Klein, Natthapong Kunthawong, Omer Fleissig, Nardi Casap, David Polak*, Stella Chaushu

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    7 Scopus citations

    Abstract

    Background: The aim of the study is to examine bone healing following augmentation with allograft or β-tricalcium phosphate (β-TCP) and evaluate orthodontic tooth movement (OTM) into the augmented sites. Methods: The study included two parts. Part I included the alveolar bone regeneration model. Osseous defects were created by extraction of the maxillary first molars in C57BL/6 mice, and the sockets were filled with allograft, β-TCP, or left unfilled (n = 6/group). Mouse allograft was prepared by a novel method using long bones. Maxillae were collected at 2, 4, and 6 weeks for microcomputed tomography (μCT) and histological analysis. In Part II, OTM was performed after full bone healing, through grafted and unfilled sockets (n = 10/group), and the second molar shift was assessed using μCT. Results: Bone volume and trabeculation were reduced in β-TCP compared with allograft and non-grafted groups at 2 and 4 weeks post-grafting, but similar at 6 weeks. Graft particles could be detected at 2 weeks post-grafting for β-TCP, and at 2 and 4 weeks for allograft. Increased osteoclasts’ presence was observed in the β-TCP group at 2 and 4 weeks compared with allograft and control. OTM was similar in the two graft groups, but impaired versus the non-grafted controls. Conclusion: β-TCP and allograft induce full normal healing but alter OTM into the regenerated sites.

    Original languageAmerican English
    Pages (from-to)1067-1075
    Number of pages9
    JournalJournal of Periodontology
    Volume91
    Issue number8
    DOIs
    StatePublished - 1 Aug 2020

    Bibliographical note

    Funding Information:
    This research was supported by the Israel Science Foundation and the Cabakoff Foundation, Hebrew University, Jerusalem, Israel. All authors have contributed to, seen, and approved the final submitted version of the manuscript. We hereby declare that this is an original manuscript, has not been sent elsewhere, and that the study has been conducted according to the highest principals of animal welfare. The authors report no conflicts of interest related to this study.

    Publisher Copyright:
    © 2019 American Academy of Periodontology

    Keywords

    • bone regeneration
    • orthodontics
    • wound healing

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