TY - JOUR
T1 - The importance of cytokines analysis in the diagnosis of vitreoretinal lymphoma
AU - Frenkel, Shahar
AU - Pe'er, Jacob
AU - Kaufman, Ron
AU - Maly, Bella
AU - Habot-Wilner, Zohar
N1 - Publisher Copyright:
© 2020 Acta Ophthalmologica Scandinavica Foundation. Published by John Wiley & Sons Ltd
PY - 2020/9/1
Y1 - 2020/9/1
N2 - Purpose: To evaluate the efficacy of the different diagnostic tests for vitreoretinal lymphoma (VRL). Methods: A cohort of 150 patients with a presumed diagnosis of VRL. Vitrectomy samples were analysed for cytology, monoclonality [polymerase chain reaction (PCR)] and cytokine levels, and anterior chamber taps were analysed for cytokine levels. Vitreoretinal lymphoma (VRL) was diagnosed after confirming the clinical suspicion with vitreal or brain cytology. Results: Vitreoretinal lymphoma (VRL) was diagnosed in 78 patients. Vitreal cytology was positive for 53/132 patients (40.2%), 36/53 had positive cytology from both the eye and the brain. Additional 25 patients had positive brain cytology. Vitreal PCR for monoclonality was positive for 32/91 patients (35.2%). Vitreal cytokine levels of interleukin (IL)-10/IL-6 were >1 for 47/110 patients (43.1%). For cytology, PCR and cytokine analysis, the respective sensitivity was 73.6%, 46.0% and 81.4%, and the accuracy of the tests was 85.6%, 60.4% and 80.9%, respectively. All three tests were available for 79 patients. In this subset, for cytology, PCR and cytokine analysis the respective sensitivity was 79.5%, 41.0% and 82.1%, respectively, and the accuracy of the tests was 89.9%, 60.8% and 81.0%, respectively. Conclusion: Cytokines analysis has an important role in the diagnosis of VRL. We suggest analysing cytokines levels in all cases suspected of VRL along with cytology and PCR analysis.
AB - Purpose: To evaluate the efficacy of the different diagnostic tests for vitreoretinal lymphoma (VRL). Methods: A cohort of 150 patients with a presumed diagnosis of VRL. Vitrectomy samples were analysed for cytology, monoclonality [polymerase chain reaction (PCR)] and cytokine levels, and anterior chamber taps were analysed for cytokine levels. Vitreoretinal lymphoma (VRL) was diagnosed after confirming the clinical suspicion with vitreal or brain cytology. Results: Vitreoretinal lymphoma (VRL) was diagnosed in 78 patients. Vitreal cytology was positive for 53/132 patients (40.2%), 36/53 had positive cytology from both the eye and the brain. Additional 25 patients had positive brain cytology. Vitreal PCR for monoclonality was positive for 32/91 patients (35.2%). Vitreal cytokine levels of interleukin (IL)-10/IL-6 were >1 for 47/110 patients (43.1%). For cytology, PCR and cytokine analysis, the respective sensitivity was 73.6%, 46.0% and 81.4%, and the accuracy of the tests was 85.6%, 60.4% and 80.9%, respectively. All three tests were available for 79 patients. In this subset, for cytology, PCR and cytokine analysis the respective sensitivity was 79.5%, 41.0% and 82.1%, respectively, and the accuracy of the tests was 89.9%, 60.8% and 81.0%, respectively. Conclusion: Cytokines analysis has an important role in the diagnosis of VRL. We suggest analysing cytokines levels in all cases suspected of VRL along with cytology and PCR analysis.
KW - cytology
KW - diagnosis
KW - IL-10
KW - PCR
KW - vitreoretinal lymphoma
UR - http://www.scopus.com/inward/record.url?scp=85078754549&partnerID=8YFLogxK
U2 - 10.1111/aos.14347
DO - 10.1111/aos.14347
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C2 - 31994839
AN - SCOPUS:85078754549
SN - 1755-375X
VL - 98
SP - e668-e673
JO - Acta Ophthalmologica
JF - Acta Ophthalmologica
IS - 6
ER -