The induction of adipose conversion by bezafibrate in 3T3-L1 cells. Synergism with dibutyryl-cAMP

Ruth Brandes*, Rivka Arad, Nissim Benvenisty, Shulamit Weil, Jacob Bar-Tana

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

The induction of adipose conversion in 3T3-L1 cells by bezafibrate (Brandes, R., Hertz, R., Arad, R., Naishtat, S., Weil, S. and Bar-Tana, J. (1987) Life Sci., 40, 935-941) was enhanced by dibutyryl-cAMP as well as forskolin, theophylline or isobutylmethylxanthine added to the incubation medium together with the bezafibrate inducer. The synergistic effect of bezafibrate and dibutyryl-cAMP resulted in enhancing the expression of late markers of adipose conversion, e.g., lipid accumulation or glycerol-3-phosphate dehydrogenase activity and its mRNA. This enhanced expression of late markers was reflected in shortening the time period required for their first appearance as well as increasing their yield during the course of adipose conversion. By following the accumulation of glutamine synthetase mRNA serving as an early marker for adipose conversion, the synergistic effect of bezafibrate and dibutyryl-cAMP was already evident as early as 5 h following their addition to confluent 3T3-L1 cells. Hence, the induction of adipose conversion by bezafibrate in 3T3-L1 cells appears to involve an early event which is rate-limited by the availability of intracellular cAMP.

Original languageAmerican English
Pages (from-to)219-224
Number of pages6
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1054
Issue number2
DOIs
StatePublished - 1 Sep 1990

Bibliographical note

Funding Information:
We thank Dr. L.P. Kozak for kindly providing the glycerol-3-P dehydrogenase probe, and Dr. R.E. Miller for kindly providing the gutamine synthetase probe. This work was supported by Grant No. 1312 from the Ministry of Health of Israel.

Keywords

  • (3T3.L1 cells)
  • Adipose conversion
  • Bezafibrate
  • dibutyryl cyclic AMP

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